Nutrition, Microbiomes, and Colony Health with the Carl Hayden Lab Team (404)
What does honey bee health look like when researchers examine it from the inside out? In this episode, Jeff and Becky welcome three scientists from the USDA-ARS Carl Hayden Bee Research Center in Tucson, Arizona: Dr. Vanessa Corby-Harris, Dr. Kirk Anderson, and Dr. Mark Carroll. Their work spans insect physiology, nutrition, microbiomes, chemical ecology, Varroa, and the complicated ways multiple stressors influence colony health.
Vanessa explains why nutrition is one of the areas where beekeepers can directly influence colony health and how researchers use biomarkers—including the honey bee fat body—to connect individual bee physiology with brood production and colony performance.
Kirk describes research comparing colonies placed in diverse Conservation Reserve Program forage with colonies working canola and sunflower landscapes, then discusses a more recent case involving Varroa, amitraz resistance, viruses, nutrition, and agricultural exposure.
Mark explores another layer: how young workers interpret colony conditions and how pheromones such as β-ocimene may influence brood rearing during nutritional stress.
Together, the conversation shows why understanding colony health increasingly means looking beyond any single stressor—and why nutrition, physiology, microbes, pesticides, pathogens, and mites often need to be considered together.
Also in this episode, Jeff and Becky answer a Hive IQ listener question about preparing plastic foundation for bees.
Websites from the episode and others we recommend:
- Carl Hayden Honey Bee Research Lab: https://www.ars.usda.gov/pacific-west-area/tucson-az/carl-hayden-bee-research-center/
- Honey Bee Health Coalition: https://honeybeehealthcoalition.org
- Project Apis m. (PAm): https://www.projectapism.org
- The National Honey Board: https://honey.com
- Honey Bee Obscura Podcast: https://honeybeeobscura.com
Copyright © 2026 by Growing Planet Media, LLC
______________

Betterbee is the presenting sponsor of Beekeeping Today Podcast. Betterbee’s mission is to support every beekeeper with excellent customer service, continued education and quality equipment. From their colorful and informative catalog to their support of beekeeper educational activities, including this podcast series, Betterbee truly is Beekeepers Serving Beekeepers. See for yourself at www.betterbee.com
This episode is brought to you by Global Patties! Global offers a variety of standard and custom patties. Visit them today at http://globalpatties.com and let them know you appreciate them sponsoring this episode!
As a beekeeper, you want products that benefit you and your bees. When you choose Premier Bee Products, you choose hive components that are healthier for bees and more productive for you. Because we believe that in beekeeping, details make all the difference. Premier Bee Products: Better for bees. Better for beekeepers. Use promo code PODCAST for 10% off your next online order.
Thanks to Strong Microbials for their support of Beekeeping Today Podcast. Find out more about their line of probiotics in our Season 3, Episode 12 episode and from their website: https://www.strongmicrobials.com
HiveIQ is revolutionizing the way beekeepers manage their colonies with innovative, insulated hive systems designed for maximum colony health and efficiency. Their hives maintain stable temperatures year-round, reduce stress on the bees, and are built to last using durable, lightweight materials. Whether you’re managing two hives or two hundred, HiveIQ’s smart design helps your bees thrive while saving you time and effort. Learn more at HiveIQ.com.

We’d like to thank Vita Bee Health for supporting the podcast. Vita provides proven tools for controlling Varroa—from Apistan and Apiguard to the new VarroxSan extended-release oxalic acid strips—helping beekeepers keep stronger, healthier colonies.
Thanks for Northern Bee Books for their support. Northern Bee Books is the publisher of bee books available worldwide from their website or from Amazon and bookstores everywhere. They are also the publishers of The Beekeepers Quarterly and Natural Bee Husbandry.

Thanks to Bee Culture, the Magazine of American Beekeeping, for their support of Honey Bee Obscura. Available in print and digital at www.beeculture.com
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We hope you enjoy this podcast and welcome your questions and comments in the show notes of this episode or: questions@beekeepingtodaypodcast.com
Thank you for listening!
Podcast music: Be Strong by Young Presidents; Epilogue by Musicalman; Faraday by BeGun; Walking in Paris by Studio Le Bus; A Fresh New Start by Pete Morse; Wedding Day by Boomer; Christmas Avenue by Immersive Music; Red Jack Blues by Daniel Hart; Bolero de la Fontero by Rimsky Music; Perfect Sky by Graceful Movement; I'm Not Running Away This Time by Max Brodie; Original guitar background instrumental by Jeff Ott.
Beekeeping Today Podcast is an audio production of Growing Planet Media, LLC
** As an Amazon Associate, we may earn a commission from qualifying purchases
Copyright © 2026 by Growing Planet Media, LLC

00:00:00.480 --> 00:00:03.040
Hey, this is Bob and this is Chris from Hive
00:00:03.280 --> 00:00:03.760
Noon.
00:00:03.760 --> 00:00:05.200
We're from Dayton, Ohio, and this
00:00:05.360 --> 00:00:07.040
welcome to beekeeping today.
00:00:07.040 --> 00:00:10.080
Welcome to Beekeeping Today podcast presented by Better
00:00:10.240 --> 00:00:10.800
Bee, your
00:00:10.960 --> 00:00:14.240
source for beekeeping news, information, and entertainment.
00:00:14.240 --> 00:00:14.960
I'm Jeff.
00:00:15.400 --> 00:00:17.080
And I'm Becky Masterman.
00:00:17.080 --> 00:00:21.880
Today's episode is brought to you by the Bee Nutrition superheroes at Global Patties.
00:00:21.880 --> 00:00:29.320
Family operated and buzzing with passion, Global Patties crafts protein-packed patties that'll turn your hives into powerhouse production.
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Picture this.
00:00:30.560 --> 00:00:35.200
Strong colonies, booming brood, and honey flowing like a sweet river.
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It's super protein for your bees and they love it.
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Check out their buffet of patties, tailor-made, for your bees in your specific area.
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Head over to
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www.
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globalpatties.
00:00:47.880 --> 00:00:50.920
com and give your bees the nutrition they deserve.
00:00:50.920 --> 00:00:54.440
Hey, a quick shout out to Betterbee and all of our sponsors
00:00:54.600 --> 00:00:59.960
whose support allows us to bring you this podcast each week without resorting to a fee-based subscription.
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We don't want that
00:01:01.760 --> 00:01:03.520
and we know you don't either.
00:01:03.520 --> 00:01:06.960
Be sure to check out all of our content on the website.
00:01:06.960 --> 00:01:14.560
There you can read up on all of our guests, read our blog on the various aspects and observations about beekeeping, search for, download, and listen to over
00:01:15.320 --> 00:01:25.080
300 past episodes, read episode transcripts, leave comments and feedback on each episode, and check on podcast specials from our sponsors.
00:01:25.080 --> 00:01:27.880
You can find it all at www.
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beekeepingtoday.
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com.
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Thank you, Bob and Chris, for that great opening from the North American Honey Bee Expo.
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You know, Becky, I used to live near Dayton, Ohio.
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I used to live in Huber Heights.
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Huber
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Heights, Ohio.
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That's a great name for a town.
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It
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it
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was
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a great place to grow up.
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You know, it's not too far from the right
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Patterson Air Force Base and I remember
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no, I'm dating myself here, but you know, that was before they restricted sonic booms.
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And the jet fighters from uh
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Wright-Patterson would fly over in the sonic booms all the time was
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uh I guess that's why I like
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thunderstorms so much.
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That's great.
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Well thank you, Mark Rifris.
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No, I want to know when they restricted sonic booms.
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I
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you know, that's a good question.
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Maybe we should look it up.
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You know, if you find that out, I'll give you a hive tool.
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Oh, that's exciting.
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Wait, do I get the hive
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tools?
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Okay, this is fair.
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This is great.
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Okay, let's move on to beekeeping.
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Beekeeping.
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Thank you, Bob and Chris.
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Appreciate it.
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Becky, it's I can't believe it's September.
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This year is just flying
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by.
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Yes,
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except there's
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there's plenty to do, right?
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Oh my god.
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We just have less time to do it.
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That is the truth.
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That is the truth.
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I still need to paint some equipment, so
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do you think I'm behind this year?
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Well, at least it's assembled.
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It's assembled.
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Yes, yes.
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But I do have some unpainted equipment out there that I need to
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um exchange with painted equipment so I can not feel super guilty
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exposing them to a Minnesota winter.
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The fall is the time when I have the greatest angst over my bees
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because
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all
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of the
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things I put off
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All of the things I neglected.
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All of the things I know I should have done but didn't do because I chose
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to do something else
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show up
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because you know the
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row is too big
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or the colony is weak
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Or
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and I'm saying every anytime, you know, bees die naturally, but every time there's a weak colony I said, I should have done something better.
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Fall
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is hard that way.
00:03:26.460 --> 00:03:30.860
It seems like you're about to write a poem called The Guilty Beekeeper.
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A woeful
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poem.
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What a great book that would be.
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It would be a
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book of poems.
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sad, morose poems.
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Nobody's buying that book.
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Yeah, no, it
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it's it's always that's the
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uh the tricky part of the year for a lot of beekeepers
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is that they're running out of time.
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as far as the nights are starting to get cooler, if they need to get feed on the bees, they only have a certain amount of time
00:03:57.240 --> 00:04:01.960
when you're looking at Varroa control, although I think it's always appropriate
00:04:02.120 --> 00:04:03.560
if you've got a problem
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the efficacy is going to change based upon how long the bees have to
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really raise those winter bees without mite pressure.
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So
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it is it's a it's a tough, tough time of year.
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And so hopefully
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All of our listeners are ahead of the game instead of behind
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s
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in getting their bees
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ready for winter, regardless of the kind of winter they get to g go through.
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Do as we say and as our guests say, don't do as we do.
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Well I'm good with Varroa control
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and feeding.
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I'm on that.
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I just have unpainted equipment, Jeff.
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That's all I'm worried about right now.
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That's on me, and I have to pay more for it if it
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if it
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I guess if I
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if it goes through too much wear and tear.
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So
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yeah.
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The bees are fine.
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The late Kim Flottum
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used to have all sorts of equipment unpainted and that's just the way it was.
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So there you go.
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Then I'm in good company
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if I
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if I don't get paint on it.
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Although I I have the paint purchased.
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It's a beautiful lilac
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color, so
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very excited about that.
00:05:01.560 --> 00:05:03.160
You'll be ready for the spring
00:05:03.320 --> 00:05:03.960
We have
00:05:04.120 --> 00:05:05.560
for our Hive IQ
00:05:05.800 --> 00:05:09.320
tool today, we have a question from Burton
00:05:09.480 --> 00:05:10.520
Scripture.
00:05:10.520 --> 00:05:15.640
Now, the Hive IQ question is a giveaway we have for our sponsor, Hive IQ.
00:05:15.100 --> 00:05:17.340
They've given us a quantity of hive
00:05:17.500 --> 00:05:19.980
tools, bright and shiny, with uh hive
00:05:20.140 --> 00:05:22.380
IQ on it and beekeeping today.
00:05:22.380 --> 00:05:26.540
And for those listeners who send us a question and we are able to answer it online
00:05:26.700 --> 00:05:26.780
,
00:05:26.940 --> 00:05:30.140
We will send that listener a brand new hive tool.
00:05:30.140 --> 00:05:31.180
So we have one for Bri
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Burton.
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Did I say Brian before?
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You know you said Burton.
00:05:36.540 --> 00:05:37.580
Are we allowed to say that
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this is a name I recognize?
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Well you know so many people and you've met so many in your
00:05:42.199 --> 00:05:42.759
in your meetings.
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Yeah.
00:05:44.520 --> 00:05:45.639
Just a few.
00:05:45.639 --> 00:05:47.479
I recognize this name
00:05:47.360 --> 00:05:48.240
What is Bert
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looking for as far as a question and an answer?
00:05:51.200 --> 00:05:51.840
He wrote us um
00:05:52.080 --> 00:05:53.760
a nice short question.
00:05:53.760 --> 00:05:55.440
It says, what is the proper
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or
00:05:56.340 --> 00:05:59.780
best way to prepare plastic frames and foundation before
00:05:59.940 --> 00:06:02.660
placing in the hive for the bees to use.
00:06:02.660 --> 00:06:02.900
So
00:06:03.060 --> 00:06:06.500
I wonder if Bert's thinking about next spring already.
00:06:06.260 --> 00:06:07.060
Do you think he's like
00:06:07.220 --> 00:06:08.420
all set for twenty
00:06:08.580 --> 00:06:11.620
twenty six and he's looking forward to twenty twenty seven?
00:06:18.699 --> 00:06:19.500
Right now.
00:06:19.500 --> 00:06:20.220
That's true.
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That would be a disaster.
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Don't do that, Bert.
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That's a good question.
00:06:25.100 --> 00:06:25.419
So
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Jeff, what do you what do you
00:06:27.380 --> 00:06:28.180
advise as
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as far as doing any prep work for plastic frames and foundation?
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If it's brand new, it should be
00:06:35.500 --> 00:06:36.220
waxed
00:06:36.620 --> 00:06:38.780
and I don't do anything extra
00:06:38.940 --> 00:06:39.900
and I use
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it.
00:06:40.700 --> 00:06:40.940
I
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I do not use plastic frames, so I don't have any direct experience with that, but I do use a good amount of plastic foundation
00:06:49.280 --> 00:06:49.599
That
00:06:49.919 --> 00:06:52.080
said, I know some people
00:06:52.400 --> 00:06:52.720
like
00:06:52.879 --> 00:06:57.599
to reuse their plastic foundation because they don't want to throw it out and put it in a landfill or
00:06:57.960 --> 00:07:02.840
whatever, and they scrub it clean, pressure wash it, and then what do you do with it?
00:07:02.840 --> 00:07:03.960
How do you prep it?
00:07:03.960 --> 00:07:07.000
And for that, the best approach is to apply
00:07:07.240 --> 00:07:08.680
a fresh coat of real
00:07:08.919 --> 00:07:09.560
beeswax
00:07:09.720 --> 00:07:11.159
using a roller or a uh
00:07:11.319 --> 00:07:12.759
brush.
00:07:12.139 --> 00:07:12.540
And then
00:07:12.699 --> 00:07:14.060
the more wax on there the
00:07:14.220 --> 00:07:14.300
the
00:07:14.540 --> 00:07:17.020
crater and the quicker the bees accept it.
00:07:17.020 --> 00:07:19.820
So are you saying hot wax then with the brush?
00:07:19.979 --> 00:07:20.860
Yes, hot wax.
00:07:20.860 --> 00:07:21.979
You melt the wax
00:07:22.060 --> 00:07:23.500
Carefully, double boiler.
00:07:23.500 --> 00:07:24.380
Like double boilers?
00:07:24.380 --> 00:07:24.780
Double boiler.
00:07:24.940 --> 00:07:25.419
You don't have any
00:07:25.580 --> 00:07:26.380
no fires.
00:07:26.380 --> 00:07:27.979
No flare-ups, please.
00:07:28.300 --> 00:07:31.180
And then you brush it on or roller it on.
00:07:31.160 --> 00:07:33.560
Onto the frame, onto the foundation.
00:07:33.560 --> 00:07:34.520
So this smells good.
00:07:34.840 --> 00:07:35.400
This smells good.
00:07:35.400 --> 00:07:36.040
It's a fun job that
00:07:36.520 --> 00:07:37.240
smelling activity.
00:07:37.240 --> 00:07:38.440
You can tell I've actually
00:07:38.680 --> 00:07:43.400
I really have never put any extra wax on foundation.
00:07:42.840 --> 00:07:44.120
And I've also never pressure
00:07:44.280 --> 00:07:45.560
washed a frame
00:07:45.800 --> 00:07:46.680
or a foundation
00:07:47.080 --> 00:07:49.400
in order to put it back in the colony.
00:07:49.400 --> 00:07:49.720
So
00:07:50.360 --> 00:07:52.440
so I'm glad you understand.
00:07:52.520 --> 00:07:53.240
If I could admit
00:07:53.720 --> 00:07:53.960
something
00:07:54.120 --> 00:07:57.320
is that I don't reuse foundation.
00:07:56.520 --> 00:07:59.080
I punch it out and I throw it away.
00:07:59.080 --> 00:08:01.160
I feel guilty, but that's just time
00:08:01.320 --> 00:08:05.880
consuming for me to pressure wash it and reuse it and melt wax and put it back on.
00:08:05.880 --> 00:08:08.440
And it's also, yeah, it's just time consuming.
00:08:08.440 --> 00:08:11.000
I just don't have the time to do that.
00:08:10.060 --> 00:08:12.460
Yeah, and it's hard, even I mean, even I th
00:08:12.620 --> 00:08:15.740
I bet some people are scraping that wax off and rendering
00:08:16.060 --> 00:08:16.380
it
00:08:16.620 --> 00:08:19.979
and I just worry about pesticides when you get to that level.
00:08:19.979 --> 00:08:23.580
If you're going to replace the foundation and it's been drawn out
00:08:23.740 --> 00:08:24.060
and
00:08:24.240 --> 00:08:24.480
and
00:08:24.640 --> 00:08:28.960
used for the last five, six years, then I'm just worried what's in that wax.
00:08:28.960 --> 00:08:29.200
So
00:08:29.440 --> 00:08:30.480
let's not judge.
00:08:30.480 --> 00:08:31.440
Let's not judge each other.
00:08:31.440 --> 00:08:31.920
How's that?
00:08:32.960 --> 00:08:34.880
So that we don't get judged.
00:08:35.339 --> 00:08:36.459
I don't want to be judged.
00:08:36.459 --> 00:08:39.899
I will say though, talking about applying wax to a foun a plastic foundation
00:08:40.139 --> 00:08:41.019
this last summer
00:08:41.180 --> 00:08:45.500
when we spoke at the North uh North Dakota Beekeepers meeting or the tri-states
00:08:45.740 --> 00:08:47.259
meeting and I stopped in at
00:08:47.420 --> 00:08:47.819
Premier
00:08:48.060 --> 00:08:48.220
Bee
00:08:48.380 --> 00:08:49.180
products
00:08:48.959 --> 00:08:50.959
And that's a short that we've produced.
00:08:50.959 --> 00:08:53.040
To see how they pre-wax
00:08:53.360 --> 00:08:57.040
thousands of sheets of foundation every day.
00:08:57.140 --> 00:08:57.940
was amazing.
00:08:57.940 --> 00:09:01.620
The machine that spits them through and it sprays them, it cools them.
00:09:01.860 --> 00:09:04.020
It just it's an amazing process.
00:09:04.020 --> 00:09:04.260
It's
00:09:04.420 --> 00:09:04.660
it's
00:09:05.140 --> 00:09:07.860
not a bunch of people with paintbrushes and rollers of
00:09:08.260 --> 00:09:08.580
hot
00:09:08.820 --> 00:09:09.860
wax.
00:09:10.339 --> 00:09:10.980
And I know that
00:09:11.139 --> 00:09:12.740
I know that a lot of places when you're ordering
00:09:12.899 --> 00:09:17.139
that wax foundation, you can order double wax or triple wax even, right?
00:09:17.139 --> 00:09:17.300
So
00:09:17.620 --> 00:09:21.060
And typically they say that the more wax on the foundation
00:09:21.259 --> 00:09:22.620
the quicker the bees accept it.
00:09:22.620 --> 00:09:24.220
So that's something to keep in mind.
00:09:24.220 --> 00:09:28.220
I just make my bees work harder and just give 'em one layer or so.
00:09:28.620 --> 00:09:29.100
Well you
00:09:29.339 --> 00:09:32.139
you also live in an area where you have great nectar flows.
00:09:32.139 --> 00:09:32.860
Yeah, yeah, they
00:09:33.019 --> 00:09:34.300
usually don't have a problem.
00:09:34.300 --> 00:09:36.060
Yeah, that's really great.
00:09:36.020 --> 00:09:38.500
Hey, today's guest, this last summer I was
00:09:38.660 --> 00:09:40.100
went to the COLOSS
00:09:40.260 --> 00:09:43.460
meeting in Pullman, Washington, and I met Dr.
00:09:43.460 --> 00:09:43.780
Vanessa
00:09:44.100 --> 00:09:44.500
Corby
00:09:44.740 --> 00:09:46.660
Harris and her team, Mark
00:09:46.940 --> 00:09:49.020
Carroll and Kirk Anderson.
00:09:49.020 --> 00:09:54.220
They were there representing the USDA-ARS Carl Hayden Bee Research Center.
00:09:54.220 --> 00:09:55.580
Fantastic people.
00:09:55.580 --> 00:09:59.740
They agreed to be on the show and talk to us about what they're doing in the lab.
00:09:59.740 --> 00:10:01.660
I'm really looking forward to talking to 'em.
00:10:01.500 --> 00:10:02.860
I love a USDA
00:10:03.020 --> 00:10:05.580
bee researcher, so this should be a lot of fun.
00:10:05.580 --> 00:10:08.700
Well we'll hear from them as soon as we get back from this short word
00:10:08.860 --> 00:10:10.140
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Bee, your
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partners in Betterbeekeeping.
00:11:00.180 --> 00:11:01.700
Hey everybody, welcome back.
00:11:01.700 --> 00:11:04.340
Sitting around this great big virtual beekeeping today
00:11:04.580 --> 00:11:05.780
podcast table.
00:11:05.780 --> 00:11:06.740
We have Dr.
00:11:06.740 --> 00:11:07.380
Vanessa
00:11:07.620 --> 00:11:09.620
Corby Harris.
00:11:09.240 --> 00:11:09.640
Dr.
00:11:09.640 --> 00:11:10.440
Kirk Anderson
00:11:10.600 --> 00:11:11.080
and Dr.
00:11:11.080 --> 00:11:13.320
Mark Carroll, all from the Carl Hayden
00:11:13.480 --> 00:11:15.080
honey bee Research Lab
00:11:15.240 --> 00:11:17.320
in Tucson, Arizona.
00:11:17.160 --> 00:11:18.920
Send some of the warmth this way.
00:11:18.920 --> 00:11:20.600
I'm glad you're here.
00:11:21.640 --> 00:11:22.920
Thank you.
00:11:23.160 --> 00:11:23.960
Welcome.
00:11:23.960 --> 00:11:27.320
This is so exciting to have the three of you here.
00:11:27.000 --> 00:11:28.520
It's going to be a great hour.
00:11:28.520 --> 00:11:34.040
Please introduce yourself and uh tell us a little bit about yourself and how you got interested in bees.
00:11:34.040 --> 00:11:37.560
And then then we'll pass the virtual mic around to everybody else.
00:11:37.420 --> 00:11:43.819
Okay, yeah, so Vanessa Corby-Harris, I'm a research insect physiologist
00:11:43.980 --> 00:11:45.259
is my, I guess
00:11:45.880 --> 00:11:48.280
formal title at the USDA-ARS lab
00:11:48.440 --> 00:11:49.800
here in Tucson.
00:11:49.800 --> 00:11:51.160
So yeah, I guess
00:11:51.320 --> 00:11:56.360
as my title sort of suggests, I have a background in insect physiology.
00:11:56.360 --> 00:11:56.680
So
00:11:56.800 --> 00:11:58.080
I got my PhD
00:11:58.240 --> 00:12:00.000
at the University of Georgia
00:12:00.240 --> 00:12:02.080
and that was in genetics
00:12:02.240 --> 00:12:04.640
and then I came to Arizona
00:12:04.820 --> 00:12:05.460
to do
00:12:05.780 --> 00:12:06.100
a
00:12:06.420 --> 00:12:08.180
postdoc fellowship
00:12:08.340 --> 00:12:09.780
working on mosquitoes
00:12:09.940 --> 00:12:10.740
actually.
00:12:10.740 --> 00:12:14.180
So we were working on mosquitoes and trying to make them
00:12:14.640 --> 00:12:16.240
less friendly to malaria
00:12:16.560 --> 00:12:18.480
using different genetic approaches.
00:12:18.480 --> 00:12:20.800
So that's where I got a lot of my
00:12:21.040 --> 00:12:24.080
true hardcore physiology training.
00:12:24.040 --> 00:12:29.959
And so that was uh a postdoc that was ending, gosh, I can't even remember how long ago.
00:12:29.959 --> 00:12:30.440
I guess
00:12:30.600 --> 00:12:33.000
20, that was 2010.
00:12:33.000 --> 00:12:33.320
So
00:12:33.560 --> 00:12:34.920
I was sort of
00:12:35.500 --> 00:12:43.819
In the insect world, and Kirk and I, we were postdocs together at this in this fellowship program at the University of Arizona.
00:12:44.000 --> 00:12:46.480
And I was sort of looking for my next step
00:12:46.639 --> 00:12:48.720
and Kirk said, hey, come
00:12:48.959 --> 00:12:50.480
come work with us on bees.
00:12:50.560 --> 00:12:54.639
And so that's that was actually how I got started in bees.
00:12:54.639 --> 00:12:57.120
I was actually a postdoc with Kirk for
00:12:57.440 --> 00:12:58.240
gosh, forty
00:12:58.480 --> 00:12:59.840
was it three or four or five?
00:12:59.840 --> 00:13:00.240
I don't know.
00:13:00.240 --> 00:13:01.440
It was a long time.
00:13:01.440 --> 00:13:03.920
That's how I got my start with bees.
00:13:03.920 --> 00:13:09.040
And it turned out that a lot of the skills that I had learned
00:13:09.240 --> 00:13:09.400
with
00:13:09.640 --> 00:13:12.680
regards to insect physiology and
00:13:13.000 --> 00:13:14.600
insect nutrition
00:13:14.920 --> 00:13:19.880
was very applicable to a lot of the questions that they were studying here.
00:13:19.880 --> 00:13:22.040
And so that was my entree into bees
00:13:22.200 --> 00:13:22.440
and
00:13:22.660 --> 00:13:28.020
So yeah, I started that in, I guess that was actually 2011 that I started at the B
00:13:28.260 --> 00:13:28.980
lab.
00:13:28.980 --> 00:13:33.220
Since 2015, I've been a scientist here with my own lab.
00:13:33.060 --> 00:13:34.580
Yeah, I've been here for a while
00:13:34.740 --> 00:13:35.460
too, got
00:13:35.620 --> 00:13:36.500
my PhD at
00:13:36.660 --> 00:13:39.540
Arizona State, then came to the U of A
00:13:39.860 --> 00:13:40.180
as
00:13:40.740 --> 00:13:42.660
Vanessa mentioned, and
00:13:43.240 --> 00:13:46.440
I was working on ants, so I'm really uh a social
00:13:46.760 --> 00:13:47.640
insect
00:13:47.960 --> 00:13:49.000
ecologist.
00:13:49.000 --> 00:13:52.920
I've done a lot of landscape work on both ants and uh
00:13:54.040 --> 00:13:59.320
and honey bees, long enough to know how horrifying landscape work can be, right?
00:13:59.880 --> 00:14:04.600
Oh let's go out in the world and do these things and then see what happens, right?
00:14:04.600 --> 00:14:05.160
But uh
00:14:05.320 --> 00:14:07.640
it's very difficult at at any rate.
00:14:07.640 --> 00:14:07.959
So
00:14:08.240 --> 00:14:12.640
Uh ironically, the ecology I work on is invisible.
00:14:12.640 --> 00:14:14.880
So I work on the honey bee microbiome
00:14:15.120 --> 00:14:18.320
and largely studying health and disease here
00:14:18.740 --> 00:14:18.900
So
00:14:19.060 --> 00:14:23.620
really it's just uh, you know, bacteria shared among niches and
00:14:23.860 --> 00:14:25.300
developmental states
00:14:25.460 --> 00:14:28.500
and queens and larvae and workers
00:14:28.660 --> 00:14:28.980
and
00:14:29.240 --> 00:14:29.400
how
00:14:29.560 --> 00:14:33.720
that influences their survival and their disease susceptibility.
00:14:33.720 --> 00:14:36.120
So I've studied, you know, the queen microbiome
00:14:36.360 --> 00:14:37.880
in extensively.
00:14:37.880 --> 00:14:39.160
This work only studies
00:14:39.480 --> 00:14:40.360
really started,
00:14:40.520 --> 00:14:43.960
Vanessa was in on the beginning of this work.
00:14:43.339 --> 00:14:45.420
and did just meticulous work.
00:14:45.420 --> 00:14:48.779
This is when I got to learn what a meticulous researcher of
00:14:48.940 --> 00:14:49.420
Vanessa
00:14:49.660 --> 00:14:49.819
is
00:14:49.980 --> 00:14:50.300
and
00:14:50.459 --> 00:14:52.540
really strives for perfection and
00:14:52.779 --> 00:14:54.220
just fantastic work.
00:14:54.220 --> 00:14:55.420
But the uh yeah, we
00:14:55.579 --> 00:14:57.660
moved into a number of different
00:14:58.000 --> 00:15:02.720
areas, plant compounds that affect, you know, host microbial health.
00:15:02.720 --> 00:15:02.880
Um
00:15:03.360 --> 00:15:04.240
Vanessa, we
00:15:04.399 --> 00:15:06.639
looked at short chain fatty acids and Vanessa
00:15:06.800 --> 00:15:07.839
's become like the
00:15:08.380 --> 00:15:08.940
the uh
00:15:09.260 --> 00:15:11.660
main component of fatty acid research
00:15:11.820 --> 00:15:12.060
uh
00:15:12.380 --> 00:15:13.500
tell you a lot more
00:15:13.740 --> 00:15:16.380
and all sorts of different fats
00:15:17.120 --> 00:15:19.040
I'm not sure how to say it correctly.
00:15:19.040 --> 00:15:20.560
I guess you can tell us later.
00:15:20.560 --> 00:15:23.920
Yeah, we've worked out a lot of different things from diet to
00:15:24.160 --> 00:15:25.279
to disease
00:15:25.519 --> 00:15:25.839
and
00:15:26.440 --> 00:15:27.080
And now
00:15:27.240 --> 00:15:27.320
I
00:15:27.480 --> 00:15:27.560
'm
00:15:27.720 --> 00:15:28.440
with Mark
00:15:28.600 --> 00:15:29.080
we're
00:15:29.240 --> 00:15:32.200
I'm working on the Varroa mite
00:15:32.360 --> 00:15:32.520
because
00:15:32.680 --> 00:15:34.840
it seems to be a problem in beekeeping.
00:15:34.840 --> 00:15:36.600
I don't know if you've heard about it
00:15:36.640 --> 00:15:36.800
But
00:15:40.080 --> 00:15:41.440
everyone's afraid of trophy
00:15:41.840 --> 00:15:42.080
laps
00:15:42.240 --> 00:15:44.400
now coming in, which it probably
00:15:44.720 --> 00:15:47.920
will, given the tight ship that's being run
00:15:48.320 --> 00:15:49.680
country to country.
00:15:49.680 --> 00:15:50.640
But yeah,
00:15:50.800 --> 00:15:52.320
I think that kind of
00:15:52.480 --> 00:15:54.960
is good for an introduction.
00:15:54.960 --> 00:15:55.760
Mark?
00:15:55.760 --> 00:15:56.800
If I can go ahead, um
00:15:56.960 --> 00:15:58.320
yeah, I'm Mark Carroll.
00:15:58.320 --> 00:16:00.240
I'm a research entomologist
00:16:00.480 --> 00:16:01.120
here.
00:16:00.959 --> 00:16:02.080
at uh Carl Hayden
00:16:02.240 --> 00:16:02.560
Bee.
00:16:02.560 --> 00:16:03.519
Research Center.
00:16:03.519 --> 00:16:08.880
Originally I came out of Mae Berenbaum's lab, uh, University of Illinois for my PhD
00:16:09.440 --> 00:16:11.279
and uh made my way to
00:16:11.900 --> 00:16:13.180
CMAVE, which is a U
00:16:13.420 --> 00:16:17.100
a USDA facility out in Gainesville, Florida, and they
00:16:17.340 --> 00:16:19.100
had some exciting adventures.
00:16:19.100 --> 00:16:19.500
Um we
00:16:19.660 --> 00:16:20.700
looked at plant uh
00:16:20.940 --> 00:16:22.380
plant compounds
00:16:22.820 --> 00:16:27.460
Now compounds that induce plant defenses, things found in caterpillar
00:16:27.620 --> 00:16:28.660
spit and so forth
00:16:28.820 --> 00:16:30.260
that turn on the full
00:16:30.839 --> 00:16:31.959
The full range.
00:16:31.959 --> 00:16:33.160
And uh when I was there
00:16:33.319 --> 00:16:38.680
we did find inceptins, which is one of the main ways that beans of all kinds
00:16:38.920 --> 00:16:39.240
detect
00:16:39.399 --> 00:16:39.560
or
00:16:39.720 --> 00:16:42.199
under attack and respond to things
00:16:42.520 --> 00:16:45.000
I did, however, this was at the height uh
00:16:45.160 --> 00:16:46.760
around 2008
00:16:47.080 --> 00:16:51.960
of all the problems that we were having with bees in the great expansion.
00:16:51.600 --> 00:16:51.839
And
00:16:52.000 --> 00:16:55.759
like so many other people, I was given an opportunity to jump into bees.
00:16:55.759 --> 00:16:56.560
Peter Teel
00:16:56.720 --> 00:17:00.639
memorably said to me, he goes, Mark, do you want to go out there and learn
00:17:00.800 --> 00:17:00.959
you know
00:17:01.120 --> 00:17:02.319
work on bees and grow it?
00:17:02.319 --> 00:17:03.120
And I said, Um
00:17:03.360 --> 00:17:05.360
it's it's a fascinating uh field
00:17:05.520 --> 00:17:06.240
and he goes
00:17:06.260 --> 00:17:08.579
go out and get stung and do something, you know.
00:17:08.579 --> 00:17:09.299
And that was
00:17:09.539 --> 00:17:10.740
that was pretty much uh
00:17:10.900 --> 00:17:12.100
my introduction.
00:17:12.100 --> 00:17:15.939
I was just down the road from Jamie Ellis, so I had some great help with
00:17:16.179 --> 00:17:18.020
collaboration with them and Jerry
00:17:18.179 --> 00:17:20.179
Hayes and some of the other folks
00:17:20.140 --> 00:17:21.260
to get me going.
00:17:21.260 --> 00:17:28.540
And then I came over here to Carl Hayden, mainly to work on nutritional ecology, looking at nutritional effects, uh
00:17:28.700 --> 00:17:31.260
seasonal effects, effects the diet.
00:17:31.060 --> 00:17:31.220
with
00:17:31.380 --> 00:17:32.260
a little bit of a chemical
00:17:32.420 --> 00:17:32.900
ecology
00:17:33.140 --> 00:17:33.540
spin
00:17:33.780 --> 00:17:35.300
and chemistry.
00:17:35.300 --> 00:17:39.300
And it's been many things since then, but I've kind of gotten back
00:17:39.260 --> 00:17:40.780
to my roots uh lately.
00:17:40.780 --> 00:17:46.299
We're still doing chemical ecology looking at things like seasonal effects, uh overwintering.
00:17:46.299 --> 00:17:47.340
These are big problems.
00:17:47.340 --> 00:17:48.860
I don't think I have to sell that hop
00:17:49.020 --> 00:17:50.620
that one too hard.
00:17:50.620 --> 00:17:50.860
But
00:17:51.100 --> 00:17:54.380
some of the other big questions that I'm interested in are
00:17:54.540 --> 00:17:57.340
how colonies manage to coordinate together
00:17:57.660 --> 00:17:57.980
to
00:17:58.220 --> 00:18:03.740
work against stress, and by stress I mean pesticides, pathogens, mites
00:18:03.940 --> 00:18:07.700
poor nutrition, the whole nine yards, the big peas that they talk about.
00:18:07.700 --> 00:18:08.340
And also
00:18:08.580 --> 00:18:08.899
how
00:18:09.139 --> 00:18:09.779
how things
00:18:09.940 --> 00:18:11.700
uh can come apart and fail.
00:18:11.700 --> 00:18:14.179
And is there anything that we can do in particular
00:18:14.340 --> 00:18:17.539
to nudge them in a direction, give them support
00:18:17.560 --> 00:18:25.720
I'll come back to that, I think, a little later with this uh β-ocimene, which is kind of on the frontier of looking at how we might be able to improve uh
00:18:25.880 --> 00:18:28.120
brood rearing at times when
00:18:28.279 --> 00:18:28.440
the
00:18:28.600 --> 00:18:28.919
colony
00:18:29.159 --> 00:18:30.440
is actually under
00:18:30.600 --> 00:18:31.559
nutritional stress
00:18:31.720 --> 00:18:32.679
during dearth.
00:18:32.679 --> 00:18:34.279
It's kind of a fascinating topic
00:18:34.440 --> 00:18:38.519
and it's the work of many other people working with me as well.
00:18:38.540 --> 00:18:40.860
Well, we're gonna need three more hours.
00:18:40.860 --> 00:18:43.500
So everybody clear your schedules.
00:18:43.500 --> 00:18:44.860
This is gonna be a multi-part
00:18:45.020 --> 00:18:45.500
segment.
00:18:45.500 --> 00:18:45.580
Or
00:18:45.820 --> 00:18:46.220
multi-part
00:18:46.700 --> 00:18:47.420
episode.
00:18:48.060 --> 00:18:48.300
Oh
00:18:48.460 --> 00:18:48.620
well
00:18:48.780 --> 00:18:49.580
this is fantastic
00:18:49.740 --> 00:18:50.300
and this
00:18:50.460 --> 00:18:52.300
I'm not even sure where to start.
00:18:52.300 --> 00:18:53.260
Me too.
00:18:53.260 --> 00:18:53.660
Yeah, it
00:18:53.820 --> 00:18:54.460
it's it's
00:18:54.620 --> 00:18:55.580
I mean I'm I'm looking
00:18:55.740 --> 00:18:57.900
uh actually I see Kirk's table in the background
00:18:58.060 --> 00:18:58.140
is
00:18:58.380 --> 00:19:00.140
all these rocks and it just made me think of
00:19:00.300 --> 00:19:00.540
all
00:19:00.860 --> 00:19:02.460
there's so many things to choose from.
00:19:02.460 --> 00:19:03.900
Where do you start?
00:19:04.419 --> 00:19:05.780
Well I have guitars.
00:19:05.780 --> 00:19:09.059
I have guitars at home, but I can't bring 'em to work.
00:19:10.660 --> 00:19:10.900
Uh
00:19:11.059 --> 00:19:12.020
the pleasure of working
00:19:12.179 --> 00:19:12.820
from home.
00:19:13.480 --> 00:19:15.799
You just haven't set up the right experiment, Kirk.
00:19:15.799 --> 00:19:16.039
If you
00:19:16.200 --> 00:19:19.720
actually set up the right experiment, you'd have guitars at work.
00:19:20.039 --> 00:19:21.000
That is correct.
00:19:21.000 --> 00:19:23.400
Well they have an acoustic room in the basement.
00:19:23.400 --> 00:19:25.720
It's completely soundproof.
00:19:25.440 --> 00:19:25.519
W
00:19:25.919 --> 00:19:27.600
nobody really knows why it's there.
00:19:27.600 --> 00:19:29.360
We have our suspicions.
00:19:29.840 --> 00:19:30.159
But
00:19:30.880 --> 00:19:31.039
they
00:19:31.200 --> 00:19:33.840
can't hear you scream in space, is that what you're saying?
00:19:33.840 --> 00:19:34.080
Yeah.
00:19:37.120 --> 00:19:38.960
Okay, on another topic.
00:19:38.960 --> 00:19:39.760
What's the
00:19:39.860 --> 00:19:43.860
The big project, what are you most excited about that you're working on right now?
00:19:43.940 --> 00:19:45.779
I mean, I guess the problem is
00:19:45.940 --> 00:19:47.139
you could kind of get
00:19:47.380 --> 00:19:49.059
from our description of what we do
00:19:49.220 --> 00:19:52.019
is we're excited about everything, unfortunately.
00:19:52.019 --> 00:19:52.340
So
00:19:52.400 --> 00:19:52.720
So
00:19:52.880 --> 00:19:53.680
really it's a
00:19:53.840 --> 00:19:54.560
it's a ex
00:19:54.800 --> 00:19:58.080
it's an exercise in restraint in many ways
00:19:58.400 --> 00:19:58.720
because
00:19:59.040 --> 00:20:02.800
we have to focus on the things that we think are most important.
00:20:02.820 --> 00:20:03.140
So
00:20:03.380 --> 00:20:03.940
my my
00:20:04.260 --> 00:20:04.580
love
00:20:04.740 --> 00:20:08.340
is insect nutrition and insect physiology.
00:20:08.340 --> 00:20:10.020
So I'll start out with that.
00:20:10.020 --> 00:20:12.260
So I think that nutrition is
00:20:12.740 --> 00:20:14.580
one of the few things
00:20:14.740 --> 00:20:15.060
that
00:20:16.080 --> 00:20:16.640
I guess
00:20:16.880 --> 00:20:18.480
we can do something about.
00:20:18.480 --> 00:20:18.640
So
00:20:18.880 --> 00:20:20.559
bee nutrition.
00:20:20.559 --> 00:20:22.960
The topics that I look at are
00:20:23.120 --> 00:20:25.440
number one, what do bees need?
00:20:25.519 --> 00:20:27.039
What nutrients do they need?
00:20:27.039 --> 00:20:29.120
Number two, how do those needs change
00:20:29.360 --> 00:20:37.039
depending on different factors like where they're located, the time of year, different stressors that they're coming into contact with?
00:20:37.039 --> 00:20:39.200
It could be anything from pesticide
00:20:39.340 --> 00:20:39.500
to
00:20:39.820 --> 00:20:42.539
mites, disease, things like that.
00:20:42.539 --> 00:20:43.660
And the third one is
00:20:43.820 --> 00:20:49.580
once you have an idea of what they need and how those needs change due to those certain factors.
00:20:49.620 --> 00:20:51.140
How do you give those
00:20:51.300 --> 00:20:53.220
those things that are missing
00:20:53.540 --> 00:20:55.060
back to the bees?
00:20:55.060 --> 00:20:55.700
And so
00:20:56.020 --> 00:21:01.780
the two main ways that you can do that is number one by giving them access to
00:21:02.260 --> 00:21:04.580
uh you know, a diverse type of forage
00:21:04.900 --> 00:21:08.100
where they can get many different types of nectars
00:21:08.340 --> 00:21:09.620
and polls.
00:21:09.620 --> 00:21:10.980
That's always the best
00:21:11.300 --> 00:21:12.420
thing, right?
00:21:12.420 --> 00:21:13.860
Because it's natural.
00:21:13.860 --> 00:21:17.460
And bees are really good at collecting that stuff from plants.
00:21:17.860 --> 00:21:24.580
And then the second way though, because a lot of times stuff is not available on the landscape either due to
00:21:25.320 --> 00:21:27.240
Things like bad weather
00:21:27.480 --> 00:21:33.240
or the way that agriculture is nowadays, where we have monocropping systems, that would be
00:21:33.400 --> 00:21:33.640
we
00:21:33.880 --> 00:21:36.920
we can give it back to them using diets.
00:21:36.900 --> 00:21:37.140
So
00:21:37.380 --> 00:21:38.420
supplements.
00:21:38.420 --> 00:21:41.620
The reason why I think that is so important
00:21:42.500 --> 00:21:47.460
is just basically the reality of the system now that we're working with.
00:21:47.460 --> 00:21:47.780
So
00:21:48.260 --> 00:21:50.340
There's a lot of stuff that we have no control
00:21:50.500 --> 00:21:50.980
over.
00:21:50.980 --> 00:21:53.700
Obviously, we don't have any control over weather.
00:21:53.700 --> 00:22:00.419
I don't think that I personally am going to be changing the way that modern agriculture is nowadays.
00:22:00.940 --> 00:22:04.460
And so, you know, we're left with those things that we can control
00:22:04.940 --> 00:22:05.260
and
00:22:05.580 --> 00:22:11.659
things like, you know, can we provide them different types of diets that are improved?
00:22:11.440 --> 00:22:13.039
compared to what we have now.
00:22:13.039 --> 00:22:15.600
I don't want to develop an entirely new diet.
00:22:15.600 --> 00:22:19.039
I think there's a lot of stuff on the market that is a good starting place
00:22:19.500 --> 00:22:20.299
But essentially
00:22:20.539 --> 00:22:26.299
can we add little components to that might kind of give them a nudge in a positive direction?
00:22:26.380 --> 00:22:29.580
The way that you evaluate whether bees are well
00:22:29.740 --> 00:22:34.059
fed is going to be different than uh how a beekeeper would do it.
00:22:34.500 --> 00:22:34.740
So
00:22:34.899 --> 00:22:35.220
how do
00:22:35.460 --> 00:22:39.220
how did you connect your research to the beekeepers?
00:22:39.220 --> 00:22:39.539
Because
00:22:39.700 --> 00:22:43.860
I think it's hard for especially for newer beekeepers to say, oh, this colony.
00:22:43.779 --> 00:22:44.019
has a
00:22:44.179 --> 00:22:45.779
has a nutrition deficit.
00:22:45.779 --> 00:22:47.620
Could you expand on that a little bit?
00:22:47.620 --> 00:22:48.260
Yeah, yeah.
00:22:48.260 --> 00:22:50.580
Thanks for giving me that opportunity.
00:22:50.580 --> 00:22:50.899
So
00:22:51.380 --> 00:22:53.620
essentially it's really hard to tell
00:22:53.779 --> 00:22:55.940
just based on the adults.
00:22:55.720 --> 00:22:55.880
what
00:22:56.360 --> 00:22:59.320
you know the adults they kind of all look the same
00:22:59.640 --> 00:22:59.880
so
00:23:00.040 --> 00:23:01.480
so it's not the adults
00:23:01.720 --> 00:23:04.920
you have to open up the hive to essentially see
00:23:05.240 --> 00:23:06.760
you know what's going on
00:23:06.880 --> 00:23:12.880
So it's gonna be things like the boot area is smaller than it should be at that particular time of year.
00:23:12.880 --> 00:23:13.760
I think that
00:23:14.160 --> 00:23:14.720
you know
00:23:14.960 --> 00:23:21.040
We have to learn from each other in terms of what would be typical for a colony at that particular location
00:23:21.200 --> 00:23:22.720
at that time of year.
00:23:22.720 --> 00:23:25.040
So, you know, that's going to be different.
00:23:24.940 --> 00:23:30.139
depending on location, but things like do they have brood, do they have all the stages of
00:23:30.380 --> 00:23:33.580
open and sealed brood, those sorts of things?
00:23:33.580 --> 00:23:38.139
Do they have stores at times of year where they should have stores
00:23:38.740 --> 00:23:42.820
Especially going into winter, you want to have plenty of pollen
00:23:43.140 --> 00:23:43.460
and
00:23:43.780 --> 00:23:45.299
nectar in the hive
00:23:45.620 --> 00:23:46.340
so that they're
00:23:46.820 --> 00:23:49.059
set up for success going in.
00:23:49.340 --> 00:23:53.820
And I mean I guess the one thing you can tell from the adults is just look at the hive entrance.
00:23:53.820 --> 00:23:57.500
Are they bringing anything in when they should be bringing things in?
00:23:57.500 --> 00:23:59.260
Those are important.
00:23:59.260 --> 00:23:59.900
Now
00:24:00.780 --> 00:24:04.860
We do measure those things in our in our studies.
00:24:04.860 --> 00:24:11.580
However, a lot of my earlier work, especially you know, when we bring up topics like physiology.
00:24:11.760 --> 00:24:16.240
That's kind of a really weird term for folks that are not familiar with it.
00:24:16.240 --> 00:24:19.200
And I just use it very casually, but
00:24:19.520 --> 00:24:21.760
but the reason why is because
00:24:22.880 --> 00:24:25.840
Every bee has their own way
00:24:26.160 --> 00:24:26.480
of
00:24:26.720 --> 00:24:29.280
dealing with what's coming into their bodies
00:24:29.440 --> 00:24:33.040
and how they're going to give that back to their hives.
00:24:33.120 --> 00:24:34.160
And essentially
00:24:34.320 --> 00:24:40.800
how they manage that input and output ratio, that's going to end up manifesting in things like brood
00:24:41.040 --> 00:24:46.080
Okay, it's gonna end up manifesting in the amount of nutrient storage that there have.
00:24:46.080 --> 00:24:48.080
So for example, the nurse
00:24:48.240 --> 00:24:48.720
fees
00:24:49.120 --> 00:24:52.560
The young nest bees, they're the ones that
00:24:52.720 --> 00:24:54.880
eat you know most of the pollen
00:24:55.120 --> 00:24:56.720
coming into the colony
00:24:56.860 --> 00:25:03.740
Now, if the queen is laying a bunch of eggs and they're rearing a bunch of brood, it might be normal to not see that much pollen
00:25:03.899 --> 00:25:04.539
stored
00:25:04.400 --> 00:25:07.120
but you're going to see a lot of brood, okay?
00:25:07.120 --> 00:25:09.360
And it's going to be of all different life stages
00:25:09.520 --> 00:25:14.480
because those nurse bees turn that pollen, they metabolize it.
00:25:14.679 --> 00:25:15.000
And
00:25:15.159 --> 00:25:17.080
they make it into brood food.
00:25:17.080 --> 00:25:27.080
Now, as foragers, they get older, they're, you know, they start out as nurse bees and then they work their way up in age and they turn into foragers.
00:25:27.019 --> 00:25:28.220
Bees that are
00:25:28.460 --> 00:25:29.899
malnourished
00:25:30.220 --> 00:25:30.539
in
00:25:32.460 --> 00:25:35.820
their younger lives, they're obviously not going to be doing so well
00:25:35.980 --> 00:25:37.899
later on in their lives.
00:25:37.919 --> 00:25:38.240
So
00:25:38.559 --> 00:25:41.120
that might turn out to be things like
00:25:41.280 --> 00:25:46.320
you're not they're not foraging for the same amount of resources as you would expect.
00:25:46.320 --> 00:25:48.480
So they're not collecting the stuff that they might
00:25:48.640 --> 00:25:50.880
that they should at that time of year
00:25:50.760 --> 00:25:53.720
And all of this basically comes back to the
00:25:53.960 --> 00:25:56.520
how the individual managed that
00:25:56.679 --> 00:26:00.039
the nutrients coming in and the nutrients going out.
00:26:00.120 --> 00:26:00.440
And
00:26:00.680 --> 00:26:06.920
that individual has to, I mean, when we say manage those things, that individual has to make
00:26:07.080 --> 00:26:09.240
a lot of choices in the hive
00:26:09.340 --> 00:26:11.179
You know, are they feeding brood?
00:26:11.179 --> 00:26:12.620
Are they supporting the queen?
00:26:12.620 --> 00:26:13.500
Things like that.
00:26:13.500 --> 00:26:16.539
So, but to answer your question briefly
00:26:20.100 --> 00:26:20.419
That
00:26:20.659 --> 00:26:22.500
input to output ratio
00:26:22.659 --> 00:26:26.899
and how those bees individually deal with those nutrients
00:26:27.040 --> 00:26:32.800
Whether or not that is successful is going to come out in things like brood and food stores.
00:26:32.800 --> 00:26:33.920
And that's what the beekeeper
00:26:34.160 --> 00:26:34.800
can see
00:26:34.740 --> 00:26:38.660
You're using a series of like biomarkers, aren't you, to m
00:26:38.820 --> 00:26:40.340
make measurements.
00:26:40.340 --> 00:26:42.100
Could you briefly
00:26:42.420 --> 00:26:43.060
share
00:26:43.380 --> 00:26:43.700
that
00:26:44.020 --> 00:26:44.740
information?
00:26:45.060 --> 00:26:46.660
Yeah, absolutely.
00:26:46.620 --> 00:26:46.940
So
00:26:47.100 --> 00:26:51.340
one of the biomarkers that we use a lot these days is fat
00:26:51.580 --> 00:26:53.020
body content.
00:26:53.180 --> 00:26:59.340
That's another term that we use that maybe not everybody knows about, but uh the fat body is essentially
00:26:59.500 --> 00:27:01.260
it does a lot of stuff in insects.
00:27:01.900 --> 00:27:02.220
It
00:27:02.460 --> 00:27:03.100
stores
00:27:03.340 --> 00:27:11.500
nutrients, so it stores protein that the individual consumes, it stores lipids or fats that the individual consumes.
00:27:11.620 --> 00:27:12.660
It's also
00:27:12.980 --> 00:27:15.620
a really key tissue for how that
00:27:15.780 --> 00:27:21.380
uh the physiology, sorry to use that term again, the physiology of the bee.
00:27:21.380 --> 00:27:26.100
So it produces things like antimicrobial peptides that might help
00:27:26.320 --> 00:27:26.480
the
00:27:26.640 --> 00:27:29.920
the bee to fight infection, things like that.
00:27:29.920 --> 00:27:30.240
So
00:27:30.480 --> 00:27:35.120
when we're looking at the fat body, we take that individual bee
00:27:35.780 --> 00:27:36.100
And
00:27:36.740 --> 00:27:37.060
we
00:27:37.380 --> 00:27:40.740
unfortunately have to collect them from the colony
00:27:40.980 --> 00:27:41.300
and
00:27:41.620 --> 00:27:43.220
we dissect them
00:27:43.760 --> 00:27:44.080
and
00:27:44.399 --> 00:27:45.919
we can measure the
00:27:46.240 --> 00:27:47.360
amount of
00:27:47.600 --> 00:27:47.840
say
00:27:48.000 --> 00:27:50.799
lipids and proteins in those bees.
00:27:50.799 --> 00:27:51.120
That
00:27:51.279 --> 00:27:55.200
is a good measure of, that's a good biomarker
00:27:55.360 --> 00:27:57.760
for how healthy the bees are.
00:27:57.620 --> 00:28:07.220
And the reason why that is, based on the individual but also the colony, is because it turns out that it's those proteins that are stored.
00:28:07.580 --> 00:28:11.980
That are recycled back to make things like brood food.
00:28:11.980 --> 00:28:13.660
It's those lipids
00:28:13.980 --> 00:28:14.299
that
00:28:14.460 --> 00:28:19.900
the bee needs to have energy to do all the things it needs to do in the colony.
00:28:19.960 --> 00:28:26.600
So that's one of those biomarkers that does a really good job of linking these kind of abstract topics
00:28:26.920 --> 00:28:31.080
in insect physiology to how a colony might look.
00:28:31.040 --> 00:28:32.080
Well, that's really good.
00:28:32.080 --> 00:28:32.480
The fat
00:28:32.640 --> 00:28:34.560
body has received a lot of press in the last eight
00:28:34.800 --> 00:28:36.240
last ten years, I would suppose.
00:28:36.560 --> 00:28:39.040
Maybe I don't know the exact number, but it's
00:28:39.100 --> 00:28:42.620
I know when I started in beekeeping, you talk about fat bodies, you're talking about beekeeper
00:28:42.780 --> 00:28:43.980
Bob, but you're not
00:28:44.299 --> 00:28:44.460
fat
00:28:44.620 --> 00:28:51.340
bodies now and beekeeping means something completely different, and that's really fascinating to hear how it's being used as a biomarker.
00:28:51.260 --> 00:28:52.700
So let's take a quick break.
00:28:52.700 --> 00:28:54.940
We're right at the time and we'll hear from our sponsors.
00:28:55.020 --> 00:28:59.580
We'll come back and we'll hear from Kirk.
00:28:59.580 --> 00:29:02.059
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Welcome back, everybody.
00:30:31.460 --> 00:30:39.380
Kirk, we're gonna we're gonna throw that same question, not about biomarkers as much, but but as far as what research are you
00:30:39.620 --> 00:30:41.940
doing right now that's exciting
00:30:42.100 --> 00:30:43.060
and you're
00:30:43.300 --> 00:30:43.620
really
00:30:44.300 --> 00:30:45.820
looking forward to
00:30:46.140 --> 00:30:48.860
seeing how it can change beekeeping.
00:30:49.100 --> 00:30:49.820
This is really
00:30:49.980 --> 00:30:50.140
oh
00:30:50.460 --> 00:30:53.020
well I'm gonna I'm gonna return to the marker-based uh
00:30:53.180 --> 00:30:54.460
physiology of
00:30:54.740 --> 00:30:54.900
Oh
00:30:55.060 --> 00:30:56.180
fantastic.
00:30:56.180 --> 00:30:56.260
But
00:30:56.420 --> 00:30:56.980
Vanessa
00:30:57.140 --> 00:30:58.260
was talking about uh
00:30:58.580 --> 00:30:59.140
That's great.
00:30:59.140 --> 00:31:00.020
So I'm reminded
00:31:00.180 --> 00:31:03.380
of a study we did in two thousand fourteen and fifteen
00:31:03.540 --> 00:31:03.860
and uh
00:31:04.020 --> 00:31:04.340
Vanessa
00:31:04.740 --> 00:31:06.500
was involved in this as well.
00:31:06.500 --> 00:31:07.540
So we went up
00:31:07.960 --> 00:31:19.480
And I've been reviewing this information because of what Mark and I are doing right now, where we went and collected a bunch of landscape level, you know, longitudinal data.
00:31:19.440 --> 00:31:22.799
that included Varroa and Virus and all this other
00:31:23.039 --> 00:31:24.559
scobbledygook.
00:31:24.559 --> 00:31:25.200
But this
00:31:25.360 --> 00:31:26.880
this particular example
00:31:27.039 --> 00:31:30.000
I complained about landscape ecology.
00:31:29.940 --> 00:31:33.779
But this particular example had a really stark result
00:31:34.899 --> 00:31:35.940
where we put some
00:31:36.100 --> 00:31:40.820
we put a whole bunch of colonies and canola and sunflowers.
00:31:40.740 --> 00:31:43.460
Which are wonderful plants, don't get me wrong.
00:31:43.460 --> 00:31:46.340
And then a whole bunch of colonies, uh other colonies
00:31:46.500 --> 00:31:53.620
went into just what's called the CRP land, which has these, you know, nice flowers and wonderful nutrition for
00:31:53.860 --> 00:31:55.220
for honey bees.
00:31:55.220 --> 00:31:56.419
And then we did uh
00:31:56.740 --> 00:31:58.899
we did collect a bunch of marker
00:31:59.139 --> 00:31:59.860
response
00:32:00.500 --> 00:32:03.139
over the course of four months
00:32:03.160 --> 00:32:05.320
or not four months, over the course of a year
00:32:05.640 --> 00:32:08.040
at four separate times uh during that year.
00:32:08.040 --> 00:32:11.080
So it was a longitudinal study as well.
00:32:11.080 --> 00:32:11.400
So
00:32:11.660 --> 00:32:13.900
And we've looked at it through overwintering, so
00:32:14.060 --> 00:32:14.140
that
00:32:14.460 --> 00:32:15.100
very important
00:32:15.420 --> 00:32:15.740
time
00:32:15.900 --> 00:32:17.980
when when bees die off.
00:32:17.980 --> 00:32:18.300
So
00:32:18.940 --> 00:32:19.100
uh
00:32:19.420 --> 00:32:21.180
bees are constantly
00:32:21.340 --> 00:32:22.700
challenged by
00:32:23.320 --> 00:32:25.000
Pathogens and viruses
00:32:25.240 --> 00:32:25.400
uh
00:32:25.560 --> 00:32:26.680
all the time.
00:32:26.680 --> 00:32:27.560
And so there's just
00:32:27.720 --> 00:32:28.120
viruses
00:32:28.360 --> 00:32:34.760
that are consistently in their colony, and if you don't get good nutrition, you're simply gonna succumb
00:32:35.220 --> 00:32:36.260
to these things.
00:32:36.260 --> 00:32:37.620
The same is true of I think
00:32:37.780 --> 00:32:39.220
most all organisms.
00:32:39.220 --> 00:32:42.260
If you don't get good nutrition, you're gonna succumb to things.
00:32:42.260 --> 00:32:45.380
And then the extent to which you're exposed to
00:32:45.540 --> 00:32:48.740
not only the pathogens that are in your hive
00:32:48.840 --> 00:32:57.880
But then the stuff that they spray on plants, for instance, maybe canola and sunflowers, are gonna make that much more difficult to respond from
00:32:58.039 --> 00:33:01.400
because some of the same systems in your body that respond
00:33:01.640 --> 00:33:01.960
uh
00:33:02.340 --> 00:33:02.500
to
00:33:02.660 --> 00:33:06.500
those poisons are the ones that respond to the pathogens as well s
00:33:06.820 --> 00:33:11.620
and which is something I'm learning and reading a lot about right now.
00:33:11.640 --> 00:33:14.280
There's some of these same systems within the bee
00:33:14.920 --> 00:33:15.160
are
00:33:15.320 --> 00:33:22.200
shut down by virus, and those are the systems that would respond to the poisoning uh of the pesticides.
00:33:22.200 --> 00:33:23.080
Right.
00:33:23.019 --> 00:33:23.340
Although
00:33:23.500 --> 00:33:23.580
I
00:33:23.740 --> 00:33:23.899
know
00:33:24.059 --> 00:33:24.299
no
00:33:24.460 --> 00:33:26.940
by no means an expert on that kind of thing.
00:33:26.940 --> 00:33:29.899
But yeah, we looked at these these two groups of bees
00:33:30.059 --> 00:33:31.100
that went into canola
00:33:31.260 --> 00:33:33.899
and sunflowers and bees that went into CRP land.
00:33:34.340 --> 00:33:34.419
with
00:33:34.580 --> 00:33:38.100
the really wonderful flowers and it was like night and day.
00:33:38.100 --> 00:33:41.299
The colonies that came out of the CRP
00:33:41.460 --> 00:33:41.539
land
00:33:41.700 --> 00:33:44.580
were just beautiful, just flowing over with bees.
00:33:44.580 --> 00:33:46.740
You looked at them and you gasped
00:33:46.740 --> 00:33:48.980
You know, like the angels had come down
00:33:49.140 --> 00:33:53.140
and then you look at the other colonies and they were really bad.
00:33:53.140 --> 00:33:53.300
And
00:33:53.460 --> 00:33:54.100
none of 'em.
00:33:54.100 --> 00:33:54.340
So
00:33:54.500 --> 00:33:54.820
they come
00:33:55.060 --> 00:33:56.500
they come out of the situation
00:33:56.740 --> 00:33:58.900
overwintering, and then they go into
00:33:59.140 --> 00:33:59.780
almonds
00:34:00.020 --> 00:34:01.460
generally.
00:34:00.840 --> 00:34:01.160
So
00:34:01.400 --> 00:34:08.679
really none of the bees that were in the canola and sunflower graded well for almonds, and all of those that were in the CRP
00:34:08.840 --> 00:34:09.080
land
00:34:09.320 --> 00:34:09.480
uh
00:34:09.720 --> 00:34:11.480
graded well for almonds.
00:34:11.480 --> 00:34:11.720
So
00:34:11.960 --> 00:34:14.120
The nutritional state, as Vanessa
00:34:14.280 --> 00:34:16.920
was talking about, was overwhelmingly good
00:34:17.160 --> 00:34:20.440
in those bees that had access to that good forage.
00:34:20.440 --> 00:34:21.320
The oxidative
00:34:21.480 --> 00:34:22.920
stress response.
00:34:22.659 --> 00:34:26.500
And this is relative to the canola and sunflowers, of course, Rick,
00:34:26.659 --> 00:34:27.780
was overwhelmingly
00:34:28.020 --> 00:34:33.379
significantly better than the bees that were in sunflowers and canola
00:34:33.659 --> 00:34:35.659
And so was the immune response.
00:34:35.659 --> 00:34:36.139
The immune
00:34:36.300 --> 00:34:37.100
response.
00:34:37.100 --> 00:34:39.980
So they all kind of followed the nutrition, right?
00:34:39.980 --> 00:34:41.980
Underlying the importance uh
00:34:42.139 --> 00:34:44.620
of nutrition on the landscape.
00:34:44.639 --> 00:34:44.960
Yeah, and
00:34:45.119 --> 00:34:46.000
it was very stark.
00:34:46.079 --> 00:34:46.960
It's really the
00:34:47.200 --> 00:34:48.480
most overwhelming result
00:34:48.639 --> 00:34:48.879
I've
00:34:49.039 --> 00:34:50.079
ever seen.
00:34:50.079 --> 00:34:51.919
Of course I work on the microbiota
00:34:52.079 --> 00:34:54.240
where we get all sorts of funny looking results
00:34:54.399 --> 00:34:56.319
and often we're not really sure
00:34:56.480 --> 00:34:58.000
what they mean at all.
00:34:58.079 --> 00:34:59.039
But this one
00:34:59.340 --> 00:35:01.420
This one was quite evident.
00:35:01.420 --> 00:35:01.980
All right.
00:35:01.980 --> 00:35:02.300
So
00:35:02.540 --> 00:35:06.380
anyway, lately I've been working with Mark on a uh kind of a Varroa
00:35:06.860 --> 00:35:07.500
virus
00:35:08.280 --> 00:35:09.400
watermelon
00:35:09.720 --> 00:35:10.680
question
00:35:10.920 --> 00:35:11.080
w
00:35:11.320 --> 00:35:14.280
where we followed these bees that went into watermelons.
00:35:14.280 --> 00:35:16.120
Mark went and collected all these and
00:35:16.260 --> 00:35:20.180
We were doing an entirely different experiment, but then it
00:35:20.420 --> 00:35:26.340
it kind of became apparent that we should try to investigate this group of information.
00:35:26.320 --> 00:35:26.480
about
00:35:26.640 --> 00:35:30.320
what was happening because we have big collapses the past couple of years
00:35:30.560 --> 00:35:33.520
and this particular population collapsed as well.
00:35:33.520 --> 00:35:35.120
So we decided, well
00:35:35.540 --> 00:35:40.820
We're gonna try to look at it with these markers to see what happened and we found that uh
00:35:40.980 --> 00:35:41.060
real
00:35:41.380 --> 00:35:44.820
I'm just putting this together, but we found that it collapsed.
00:35:44.820 --> 00:35:46.900
I don't know if you guys have heard about amitraz
00:35:47.140 --> 00:35:47.860
resistance
00:35:48.180 --> 00:35:48.820
probably
00:35:49.040 --> 00:35:51.280
So it turned out all the
00:35:51.440 --> 00:35:52.320
the Varroa
00:35:52.480 --> 00:35:54.080
was really high going into
00:35:54.320 --> 00:35:58.800
overwintering, and all of those Varroa were resistant to amitraz
00:35:59.540 --> 00:36:00.500
And the beekeeper
00:36:00.740 --> 00:36:01.860
used amitraz
00:36:02.180 --> 00:36:02.980
towels,
00:36:03.300 --> 00:36:04.900
so they didn't have any effect.
00:36:04.900 --> 00:36:09.940
And these bees were also in watermelons where they where they sprayed neonicotinoids
00:36:10.240 --> 00:36:10.720
and uh
00:36:10.960 --> 00:36:11.440
apparently
00:36:11.600 --> 00:36:14.080
two different kinds of fungicides, but
00:36:14.240 --> 00:36:19.360
we don't really know which ones they were using because we didn't run a chemical analysis.
00:36:19.360 --> 00:36:21.760
But all of the markers I just talked about
00:36:22.000 --> 00:36:24.080
were incredibly low
00:36:24.140 --> 00:36:27.339
in these bees going into overwinter, right.
00:36:27.339 --> 00:36:28.539
So they'd been uh
00:36:28.779 --> 00:36:29.579
what I would say
00:36:29.740 --> 00:36:33.099
exposed to the agricultural nightmare.
00:36:32.840 --> 00:36:34.360
at the very least, right?
00:36:34.360 --> 00:36:39.400
And when that happens, then you lack this ability to fight off the viruses.
00:36:39.400 --> 00:36:39.560
So
00:36:39.720 --> 00:36:41.800
the virus loads went high.
00:36:41.559 --> 00:36:42.359
The Varroa
00:36:42.520 --> 00:36:44.839
helped spread all the viruses, the deform
00:36:45.000 --> 00:36:49.559
wheat virus, and then they also have the exacerbation of
00:36:49.720 --> 00:36:55.000
whatever pesticides they're using in watermelon, which although watermelon is considered one of the
00:36:55.140 --> 00:37:00.579
the not the dirty dozen, I guess, but uh one of the cleanest, they say.
00:37:00.740 --> 00:37:02.180
I'm not really sure uh
00:37:02.500 --> 00:37:03.779
what that means.
00:37:03.779 --> 00:37:05.460
So they do this uh
00:37:06.040 --> 00:37:06.840
What do they call it?
00:37:06.920 --> 00:37:12.280
Markimagation, where they pump the chemicals in the water to irrigate it, right?
00:37:12.280 --> 00:37:15.160
With the and so the bees will collect that water
00:37:15.320 --> 00:37:15.640
too
00:37:15.800 --> 00:37:19.160
when it's hot out and so that it's just a direct
00:37:19.540 --> 00:37:22.340
A direct way of uh poisoning the bees.
00:37:22.580 --> 00:37:23.300
Would you call that
00:37:23.460 --> 00:37:24.420
chemigation?
00:37:24.580 --> 00:37:25.460
Yeah, I think that's what it's
00:37:25.780 --> 00:37:26.660
Yeah, that's the
00:37:26.900 --> 00:37:28.420
technical term for it.
00:37:28.420 --> 00:37:28.500
I
00:37:28.660 --> 00:37:29.860
never heard of that term before.
00:37:30.100 --> 00:37:30.180
Like
00:37:30.340 --> 00:37:30.660
irrigation
00:37:30.820 --> 00:37:31.300
chemigation
00:37:31.840 --> 00:37:36.720
Sorry I didn't talk about microbes, but I'm finishing a giant thing on microbes
00:37:36.880 --> 00:37:40.000
and people just get lost when I talk about microbes anyway.
00:37:40.000 --> 00:37:40.080
So
00:37:40.480 --> 00:37:42.800
I thought I'd talk about some of the stuff that
00:37:43.420 --> 00:37:48.940
that's that's more in line with the rest of what's happening, I think, in the big bee world.
00:37:48.940 --> 00:37:49.500
The bees are
00:37:49.660 --> 00:37:53.340
the beekeepers are not concerned about the microbes.
00:37:53.340 --> 00:37:55.260
They think they might want to get some
00:37:55.799 --> 00:37:58.359
probiotics that are gonna work.
00:37:58.359 --> 00:38:00.520
But we did a paper on that and we found that
00:38:00.920 --> 00:38:03.240
the probiotics really aren't doing anything.
00:38:03.240 --> 00:38:05.799
At least the ones that are popular on the market.
00:38:06.540 --> 00:38:06.940
Yeah, they
00:38:07.180 --> 00:38:08.220
they can't even be found.
00:38:08.220 --> 00:38:08.940
That's a whole nother
00:38:09.100 --> 00:38:09.740
podcast.
00:38:10.300 --> 00:38:13.500
The microbiome work I think for that needs to be a video
00:38:13.660 --> 00:38:16.220
cast so that you can show the pretty pictures of
00:38:16.380 --> 00:38:17.180
your data
00:38:17.340 --> 00:38:18.860
because those data are
00:38:19.180 --> 00:38:19.500
Are
00:38:19.660 --> 00:38:19.740
they
00:38:20.060 --> 00:38:20.859
beautiful
00:38:21.099 --> 00:38:21.420
and
00:38:21.579 --> 00:38:22.140
and um
00:38:22.460 --> 00:38:24.540
maybe artwork at the same time.
00:38:24.700 --> 00:38:26.859
Yeah, they're shocking, that's for sure
00:38:27.160 --> 00:38:27.320
So
00:38:27.560 --> 00:38:28.440
g sorry Mark I
00:38:28.600 --> 00:38:29.480
took too long.
00:38:29.480 --> 00:38:31.160
I was just gonna say it's amazing because
00:38:31.320 --> 00:38:34.280
I feel like uh you know beekeepers are dealing with uh
00:38:34.460 --> 00:38:36.860
The horses of the apocalypse at all times.
00:38:36.860 --> 00:38:39.820
You know, it's it's land, sea and air, depending on uh
00:38:40.140 --> 00:38:40.540
what's
00:38:40.700 --> 00:38:41.900
what's getting at them.
00:38:41.900 --> 00:38:44.700
That was a Queen's study that we picked up right in the middle
00:38:44.860 --> 00:38:46.780
when everything kinda came apart
00:38:46.760 --> 00:38:47.320
for this.
00:38:47.320 --> 00:38:49.000
And he was doing many things right.
00:38:49.000 --> 00:38:52.760
It just happens sometimes, uh, under the wrong conditions.
00:38:52.760 --> 00:38:55.160
I gotta admit the thing that gets me up in the morning
00:38:55.320 --> 00:38:56.120
and probably
00:38:56.640 --> 00:38:57.440
the thing that links a
00:38:57.599 --> 00:39:00.400
fair amount of the things I'm looking at is trying to figure out
00:39:00.559 --> 00:39:01.520
we have all these things.
00:39:01.520 --> 00:39:01.599
We
00:39:01.839 --> 00:39:03.200
you know I'm an ecologist.
00:39:03.200 --> 00:39:03.359
Um
00:39:03.520 --> 00:39:04.000
I would say
00:39:04.160 --> 00:39:04.799
Kirk, you know
00:39:04.960 --> 00:39:05.279
Vanessa
00:39:05.599 --> 00:39:06.240
to a certain extent
00:39:06.400 --> 00:39:07.920
are ecologists too.
00:39:07.920 --> 00:39:09.520
That means we're kind of economist
00:39:09.680 --> 00:39:11.359
of life.
00:39:10.640 --> 00:39:15.839
And we're looking at inputs, outputs, we're looking at what it actually produces
00:39:16.319 --> 00:39:20.640
and under what conditions and so forth like that, stressors, those
00:39:21.120 --> 00:39:24.560
horses of the apocalypse that are uh coming down on it.
00:39:24.560 --> 00:39:24.640
I
00:39:24.800 --> 00:39:25.040
thought
00:39:25.200 --> 00:39:25.280
one
00:39:25.440 --> 00:39:28.640
of the things that kind of uh woke me up about this was
00:39:28.880 --> 00:39:31.040
long before you kill bees
00:39:31.359 --> 00:39:33.760
You're gonna mess up how they operate
00:39:34.079 --> 00:39:35.200
for critical things.
00:39:35.200 --> 00:39:37.839
And when I say critical, it's not feels
00:39:38.000 --> 00:39:38.880
or stuff like that.
00:39:38.880 --> 00:39:42.240
It's things like brood rearing and taking care of your queen
00:39:42.520 --> 00:39:46.040
Making sure that there's a good balance as far as nutrition.
00:39:46.280 --> 00:39:48.119
You see that go out of the window with no
00:39:48.280 --> 00:39:48.760
SEMA
00:39:48.920 --> 00:39:50.359
when things are out of whack
00:39:50.599 --> 00:39:51.160
there.
00:39:50.960 --> 00:39:53.920
And the thing that's amazing about this is that
00:39:54.160 --> 00:39:57.680
this is being done by young adult bees.
00:39:57.680 --> 00:40:03.920
All these decisions that are being made for the colony are these young adult bees, they don't have much experience
00:40:04.080 --> 00:40:05.760
in their lives.
00:40:05.160 --> 00:40:08.520
And yet they're going to have to interpret whatever is coming in
00:40:08.680 --> 00:40:11.160
and hopefully make some good choices.
00:40:11.160 --> 00:40:15.240
And those choices, you know, a lot of the things we look at, you know, when their
00:40:15.640 --> 00:40:18.200
times are good, we go, those are what we want
00:40:18.160 --> 00:40:22.000
Some of the things are when things are bad, you know, not rearing brood, eating
00:40:22.160 --> 00:40:29.040
cannibalizing brood, maybe getting rid of your queen, those may be good at certain times, the beekeepers may not like those.
00:40:29.180 --> 00:40:29.500
And
00:40:29.820 --> 00:40:31.740
the one appreciation above all
00:40:31.900 --> 00:40:33.500
is that this is a long game.
00:40:33.500 --> 00:40:35.020
You know, we're we're talking
00:40:35.260 --> 00:40:35.579
I s
00:40:35.740 --> 00:40:39.740
I started out with nutrition here and I was thinking, okay, let's look at nutrition um
00:40:40.060 --> 00:40:41.740
about a month or two before they go into
00:40:41.900 --> 00:40:42.780
almonds
00:40:42.640 --> 00:40:44.559
And then that got pushed back to
00:40:44.799 --> 00:40:48.960
let's look at nutrition in the early fall before they close up.
00:40:48.960 --> 00:40:50.960
We're having indications from coloss
00:40:51.359 --> 00:40:52.160
of all things
00:40:52.319 --> 00:40:52.640
that we
00:40:52.799 --> 00:40:56.400
gotta maybe even think further back as far as impacts
00:40:56.320 --> 00:40:57.040
on things.
00:40:57.040 --> 00:40:59.520
And that's what makes it so complex.
00:40:59.520 --> 00:41:04.720
But I will say that uh a lot of what I do is uh and that's why I look at pheromones.
00:41:04.740 --> 00:41:06.180
Because what we're really trying to do is
00:41:06.340 --> 00:41:06.980
pheromones
00:41:07.140 --> 00:41:08.660
are a big part of how
00:41:08.900 --> 00:41:10.980
those bees, those young bees, uh
00:41:11.220 --> 00:41:16.340
interpret what's going on and hopefully make the right decisions for their colony.
00:41:16.140 --> 00:41:21.180
whether or not to rear brood, whether or not to rear new queen or get rid of their queen in that case.
00:41:21.180 --> 00:41:26.059
And of course these feed into major issues of what we want to do.
00:41:25.960 --> 00:41:28.360
It's okay if they do those things when
00:41:28.520 --> 00:41:31.960
they're kind of doing them in the right way, aligned with beekeepers.
00:41:31.960 --> 00:41:33.320
But I mean, for example, with brew
00:41:33.480 --> 00:41:36.520
bring, how many times have people had something in a holding yard
00:41:36.680 --> 00:41:37.000
or
00:41:37.240 --> 00:41:37.320
right
00:41:37.480 --> 00:41:39.400
before the season and they're feeding pollen
00:41:39.560 --> 00:41:40.360
patty there.
00:41:40.360 --> 00:41:43.720
And those bees are getting fat, but they're not doing squat as far as brood
00:41:43.880 --> 00:41:44.280
bearing.
00:41:44.280 --> 00:41:46.760
They're just kind of holding back on it.
00:41:46.640 --> 00:41:50.480
Or you get a queen that's perfectly good just knocked off and you're wondering
00:41:50.640 --> 00:41:50.960
why did
00:41:51.279 --> 00:41:52.000
why'd they go
00:41:52.160 --> 00:41:53.839
why'd they knock her off?
00:41:53.839 --> 00:41:56.400
So that's why I look at the these pheromones
00:41:56.720 --> 00:42:00.799
and probably the best way to think of it, particularly with the brood pheromones, is
00:42:00.859 --> 00:42:03.500
They had some French researchers, Mason
00:42:03.660 --> 00:42:08.619
Ace, uh, Cedric Aloo, some of those folks, came out with the bright and brilliant idea
00:42:08.859 --> 00:42:09.180
that
00:42:09.520 --> 00:42:10.240
Everything ha
00:42:10.400 --> 00:42:11.520
everything in the colonies
00:42:11.760 --> 00:42:14.240
far as workers and larva and stuff
00:42:14.480 --> 00:42:15.440
have a little say
00:42:15.680 --> 00:42:16.320
in how
00:42:16.480 --> 00:42:18.160
in nudging these
00:42:18.400 --> 00:42:22.960
workers as they go from in their lives from being nest bees to foragers.
00:42:23.040 --> 00:42:23.200
how
00:42:23.440 --> 00:42:29.040
fast that goes, how much effort they're going to put in, they're kind of getting influenced by these pheromones.
00:42:29.040 --> 00:42:30.080
And β-ocimene
00:42:30.240 --> 00:42:31.440
is one of those pheromones.
00:42:31.440 --> 00:42:32.800
It's made by a lot of
00:42:32.960 --> 00:42:33.440
β-ocimene
00:42:33.680 --> 00:42:35.760
is made in large amounts by eggs
00:42:36.020 --> 00:42:36.900
in very young
00:42:37.060 --> 00:42:37.940
larva.
00:42:37.940 --> 00:42:38.339
And it
00:42:38.500 --> 00:42:40.339
also doubles up as a
00:42:40.660 --> 00:42:40.820
let
00:42:40.980 --> 00:42:41.300
's mean
00:42:41.460 --> 00:42:41.619
also
00:42:41.780 --> 00:42:42.820
doubles up it's a
00:42:42.980 --> 00:42:43.300
um
00:42:43.640 --> 00:42:48.359
Essentially, it's a starvation signal and a feeding signal to workers.
00:42:48.359 --> 00:42:50.680
It's like a baby bird chirping, trying to get
00:42:51.000 --> 00:42:52.839
attention and trying to be fed.
00:42:53.539 --> 00:42:59.619
We have researchers that showed that uh older larvae put out a lot of that and they get fed more if if they
00:42:59.859 --> 00:43:01.859
if they're putting that out.
00:43:01.859 --> 00:43:02.180
So
00:43:02.840 --> 00:43:04.360
There's a lot of ideas there.
00:43:04.360 --> 00:43:08.520
I'm still interested in improving the bottom line of
00:43:08.680 --> 00:43:11.000
of what we're doing here, but pro
00:43:11.240 --> 00:43:12.680
possibly on the margin.
00:43:12.680 --> 00:43:14.920
I think the latest thing that we have
00:43:15.140 --> 00:43:17.299
With that is looking at β-ocimene
00:43:17.779 --> 00:43:19.140
and looking at the fact that
00:43:19.299 --> 00:43:22.500
something that we picked up with and we were kind of shocked.
00:43:22.500 --> 00:43:26.339
It's not something that we can see or smell very easily.
00:43:26.240 --> 00:43:27.280
But, you know, you
00:43:27.440 --> 00:43:29.360
if you happen to have a GC-MS
00:43:29.600 --> 00:43:29.760
or
00:43:30.000 --> 00:43:32.000
some other instruments just lying around which
00:43:32.480 --> 00:43:33.760
Tell everybody what that is.
00:43:34.480 --> 00:43:36.960
Mark, you have to tell everybody, not everybody here.
00:43:36.960 --> 00:43:37.760
Yeah.
00:43:37.920 --> 00:43:39.760
Just just pull it out of the garage and use it.
00:43:39.760 --> 00:43:40.480
You know, um with
00:43:40.720 --> 00:43:40.880
with
00:43:41.039 --> 00:43:41.279
that
00:43:42.340 --> 00:43:44.500
Well we noticed that we kept on getting things where
00:43:44.820 --> 00:43:46.100
wait I'm not kidding Mark.
00:43:46.100 --> 00:43:46.980
Oh no, no, I know.
00:43:47.220 --> 00:43:47.620
gas chromatography-mass spectrometry.
00:43:47.700 --> 00:43:48.500
So yeah, I'll explain
00:43:48.660 --> 00:43:48.980
whether that
00:43:49.140 --> 00:43:50.660
that's just a thing that measures uh
00:43:50.820 --> 00:43:52.820
odoriferous compounds.
00:43:52.440 --> 00:43:53.800
You put it through it separates them
00:43:53.960 --> 00:43:55.880
out, you get peaks, and you can say, Ah
00:43:56.440 --> 00:43:56.520
the
00:43:56.760 --> 00:43:59.480
the peaks are that big and I'm getting that much of it.
00:43:59.480 --> 00:44:00.920
It's another one of those instruments
00:44:01.140 --> 00:44:02.740
We talk about like everybody has one.
00:44:04.660 --> 00:44:06.500
Now I think you have one in your garage.
00:44:08.020 --> 00:44:08.260
That's
00:44:08.819 --> 00:44:09.460
a fair one there.
00:44:12.420 --> 00:44:12.980
It might have.
00:44:13.060 --> 00:44:15.060
Yeah, you got a cool 150,000.
00:44:15.060 --> 00:44:15.220
It's
00:44:15.380 --> 00:44:16.260
it's right there.
00:44:16.260 --> 00:44:16.500
Um
00:44:16.740 --> 00:44:17.300
but we were
00:44:17.460 --> 00:44:18.980
we were surprised and shocked.
00:44:18.980 --> 00:44:19.700
You get these just
00:44:20.020 --> 00:44:20.260
so
00:44:20.420 --> 00:44:22.500
stories that occur in bees.
00:44:21.900 --> 00:44:23.900
And a lot of these things with pheromones
00:44:24.140 --> 00:44:26.059
and with other stories like queen pheromones
00:44:26.220 --> 00:44:28.859
and stuff, those were made when times are good.
00:44:28.859 --> 00:44:29.819
You know, they looked at
00:44:29.980 --> 00:44:31.740
they measured queen pheromones, Winston
00:44:31.900 --> 00:44:33.099
and those folks.
00:44:32.859 --> 00:44:35.660
They figured that out when things were going well
00:44:35.900 --> 00:44:37.579
and when they were gone.
00:44:37.579 --> 00:44:38.140
Same with uh
00:44:38.380 --> 00:44:39.099
β-ocimene.
00:44:39.099 --> 00:44:42.380
It's given off a lot by these eggs and young young brood
00:44:42.619 --> 00:44:45.260
when things are healthy and when there's lots of food
00:44:45.260 --> 00:44:47.420
And we were looking at things and we noticed in the mill
00:44:47.660 --> 00:44:47.900
summer
00:44:48.060 --> 00:44:49.660
dearth when we have no forage
00:44:49.820 --> 00:44:52.300
whatsoever, they weren't giving off β-ocimene
00:44:52.540 --> 00:44:53.180
at all
00:44:53.220 --> 00:44:53.380
So
00:44:53.539 --> 00:44:55.380
we're thinking, okay, it's uh
00:44:55.700 --> 00:45:01.299
it's a bit of a starvation signal, a little bit of a baby bird chirping uh signal for older larvae.
00:45:01.299 --> 00:45:03.299
What does it do for younger larvae?
00:45:03.160 --> 00:45:09.640
And you gotta realize that younger larva, we're looking at the pivot point with brood productivity there.
00:45:09.640 --> 00:45:11.480
Because younger larvae.
00:45:11.480 --> 00:45:11.560
.
00:45:11.559 --> 00:45:16.520
When bees need to restrict the population, what they do is eat eggs and young larva.
00:45:16.520 --> 00:45:18.440
They don't eat older larva, they eat
00:45:18.599 --> 00:45:20.039
eggs and young larvae.
00:45:20.200 --> 00:45:21.400
That's a pretty definitive
00:45:21.559 --> 00:45:22.839
you're not going to get brood care
00:45:23.160 --> 00:45:26.359
kind of uh story there if you're gonna be eating.
00:45:25.980 --> 00:45:26.220
Uh
00:45:26.380 --> 00:45:26.700
so
00:45:27.180 --> 00:45:27.260
I
00:45:27.500 --> 00:45:28.060
you know, our
00:45:28.220 --> 00:45:30.540
very simple thing that we looked and we're just looking at
00:45:30.700 --> 00:45:31.500
if you supplement
00:45:31.660 --> 00:45:34.460
it in, do you get more brood rearing at these times
00:45:34.620 --> 00:45:35.100
when you
00:45:35.260 --> 00:45:40.460
You have bees, you've been feeding them lots of pollen patty, they're holding back because there's no nectar coming in.
00:45:40.460 --> 00:45:41.660
And sure enough, they
00:45:41.900 --> 00:45:43.820
in this in these cases it does.
00:45:44.060 --> 00:45:44.380
They do
00:45:44.540 --> 00:45:44.940
rear
00:45:45.180 --> 00:45:47.500
more brood through that page.
00:45:47.440 --> 00:45:50.480
You could certainly come up with situations in your mind where
00:45:50.640 --> 00:45:58.960
maybe sometime of year when you're in a forage-free area where you're feeding the daylights out of your bees with supplemental feed, you wanna you wanna push them through it.
00:45:59.020 --> 00:46:01.660
But you know, we got a long way to go to look at that.
00:46:01.980 --> 00:46:06.859
It's going to depend on nutrition again, and we know that's gonna vary place to place.
00:46:06.960 --> 00:46:11.360
Is it connected to the amount of brood food that you're going to actually visualize in a cell?
00:46:11.360 --> 00:46:15.840
There's some of it in there, but it's mainly going to be you're mainly going to find it with egg laying
00:46:16.000 --> 00:46:16.640
queens, uh
00:46:16.800 --> 00:46:21.680
eggs themselves, and then the very young larva that you have there.
00:46:21.059 --> 00:46:26.180
Uh the older larvae give off smaller amounts, but they'll give it off in burst when they're they're being ignored.
00:46:26.180 --> 00:46:26.339
You
00:46:26.500 --> 00:46:27.859
if you ever see 'em when they
00:46:28.020 --> 00:46:28.500
some of the
00:46:28.660 --> 00:46:30.180
older larvae corkscrew
00:46:30.339 --> 00:46:32.099
out and fall out of the
00:46:32.620 --> 00:46:36.060
They um need to be fed a few hundred times during the fifth
00:46:36.220 --> 00:46:37.180
instar.
00:46:37.180 --> 00:46:40.620
But if you have a lot of the β-ocimene, will you have a lot of brood
00:46:40.780 --> 00:46:41.580
food?
00:46:41.460 --> 00:46:42.100
Like if you're re
00:46:42.340 --> 00:46:46.180
you're seeing high levels, does do you see high levels of brood food?
00:46:46.180 --> 00:46:47.780
That's an excellent question.
00:46:47.780 --> 00:46:52.340
That's kind of the thing that we catch on, is that they provision more brood
00:46:52.500 --> 00:46:53.140
food.
00:46:53.760 --> 00:46:58.960
You think of your young larvae, they're just basically they're not fed little piecemeals like a baby bird.
00:46:58.960 --> 00:47:00.080
What happens is the larva
00:47:00.320 --> 00:47:02.160
the workers come in there.
00:47:01.700 --> 00:47:01.859
And
00:47:02.020 --> 00:47:04.980
you just kind of take some of those hypopharyngeal glands, those lar
00:47:05.140 --> 00:47:05.220
the
00:47:05.380 --> 00:47:06.740
brood food that Vanessa
00:47:06.980 --> 00:47:10.820
was talking about, that she measures, and they spit that up into the cell.
00:47:10.820 --> 00:47:11.060
And you
00:47:11.300 --> 00:47:14.420
there's your little larva sitting on this puddle of food
00:47:14.200 --> 00:47:17.000
It's really one of the more bizarre things about bees.
00:47:17.000 --> 00:47:18.119
And that's what they're gonna
00:47:18.440 --> 00:47:19.960
that's what they're gonna get.
00:47:19.960 --> 00:47:25.720
If they don't you know, if they don't have good glands, they're not gonna make much food, they can't rear much larvae
00:47:25.680 --> 00:47:26.720
It's not gonna be good.
00:47:26.720 --> 00:47:29.760
If they don't get that, then they're gonna starve in a few hours.
00:47:29.760 --> 00:47:30.800
And that will be that.
00:47:30.800 --> 00:47:31.600
And it'll be can
00:47:32.000 --> 00:47:35.040
they'll be recycled, I guess, back into the
00:47:35.440 --> 00:47:36.560
Food is short and
00:47:36.800 --> 00:47:40.160
yeah, it's it's gotta be kept within the colony.
00:47:40.160 --> 00:47:44.720
And it seems like different genetics might have a different
00:47:45.240 --> 00:47:45.720
threshold
00:47:45.880 --> 00:47:48.200
for cannibalizing the young.
00:47:48.200 --> 00:47:48.680
Like like
00:47:48.840 --> 00:47:53.240
I think about Carniolans and they're not afraid to cannibalize eggs
00:47:53.440 --> 00:47:53.599
if
00:47:53.760 --> 00:47:55.280
the season changes.
00:47:55.359 --> 00:47:57.119
I think unmistakably um
00:47:57.280 --> 00:47:57.920
you have
00:47:58.240 --> 00:48:01.839
you think about the Italian strains that we've selected for.
00:48:01.839 --> 00:48:04.720
We select for productivity, brood productivity like
00:48:04.880 --> 00:48:05.040
out
00:48:05.200 --> 00:48:06.000
of the gate
00:48:05.960 --> 00:48:07.640
And then we have other ones like you know
00:48:07.800 --> 00:48:08.920
Russians and
00:48:09.080 --> 00:48:09.400
and
00:48:09.560 --> 00:48:12.120
Caucasians that hold back a little bit.
00:48:12.120 --> 00:48:13.800
This is a lot of the big differences
00:48:14.040 --> 00:48:17.080
between different subspecies and uh queen
00:48:17.240 --> 00:48:19.080
stocks that we have out there
00:48:19.059 --> 00:48:22.980
There's undoubtedly different thresholds for what they're willing to put up with
00:48:23.140 --> 00:48:28.339
and how those workers interpret the forage and nutritional environment.
00:48:28.220 --> 00:48:29.500
Yeah, we do have uh
00:48:29.740 --> 00:48:32.700
I should say that just because they're making less brood
00:48:33.020 --> 00:48:34.539
doesn't mean they're they're poor.
00:48:34.539 --> 00:48:38.779
I would have to tip my hat to uh William Meikle, who is here
00:48:38.940 --> 00:48:40.940
and just recently retired
00:48:40.880 --> 00:48:46.560
And he did some work where he was looking at Russians and Italians and the Russians make less brood, but the
00:48:46.720 --> 00:48:49.440
but the workers they produce are longer lived
00:48:49.600 --> 00:48:51.600
by quite a l quite a bit.
00:48:51.640 --> 00:48:54.920
So it's a quality versus quantity thing in that case.
00:48:54.920 --> 00:48:56.760
Well Becky, when you started this and said
00:48:57.240 --> 00:48:59.319
next three hours, I I think you're
00:48:59.980 --> 00:49:00.540
So accurate.
00:49:00.780 --> 00:49:02.300
Oh I was dead serious.
00:49:05.100 --> 00:49:10.220
Make sure you invite them back now so they can commit on air and then if they don't come back, it's they're all gonna
00:49:10.380 --> 00:49:10.380
be.
00:49:14.400 --> 00:49:14.480
And
00:49:14.720 --> 00:49:14.880
say
00:49:15.039 --> 00:49:15.839
if you want to have
00:49:16.000 --> 00:49:19.760
introductions to Vanessa, Kirk, and Mark, listen to this episode.
00:49:19.760 --> 00:49:20.720
We're going to continue the
00:49:20.880 --> 00:49:23.279
discussion on this next episode.
00:49:23.279 --> 00:49:25.680
There's so much information here.
00:49:25.640 --> 00:49:28.839
I think we're only just looking at the tip of the iceberg.
00:49:28.839 --> 00:49:30.119
I wanted to ask you before we
00:49:30.359 --> 00:49:32.279
just quick answers on these, because
00:49:32.440 --> 00:49:33.880
I met you all at COLOSS
00:49:34.200 --> 00:49:35.559
in Pullman.
00:49:35.260 --> 00:49:42.140
Was there anything about what you heard from researchers around the world at that meeting that surprised you?
00:49:42.380 --> 00:49:42.460
Was
00:49:42.620 --> 00:49:43.900
there something there that was
00:49:44.059 --> 00:49:45.339
Kind of stood out.
00:49:45.339 --> 00:49:48.299
I can say it didn't surprise me, but it stood out.
00:49:48.299 --> 00:49:49.339
Yeah, no, that's great.
00:49:49.339 --> 00:49:49.900
That's fair.
00:49:49.900 --> 00:49:50.460
Yeah.
00:49:50.460 --> 00:49:56.220
It's always great when people are doing their independent research and you converge on the same answer.
00:49:56.480 --> 00:49:56.880
And I
00:49:57.200 --> 00:49:58.400
think what was really
00:49:58.560 --> 00:50:00.240
coming up a lot is how
00:50:00.560 --> 00:50:01.200
just because
00:50:01.360 --> 00:50:08.240
you have a certain species of plant that makes a certain type of nectar or pollen, it's not a static thing.
00:50:08.240 --> 00:50:09.200
It's not like
00:50:09.559 --> 00:50:10.760
you know, you measure it once
00:50:10.920 --> 00:50:13.240
and that's the nutrition of that plant.
00:50:13.240 --> 00:50:17.480
It really depends on the environment that the plant is in
00:50:17.880 --> 00:50:18.200
and
00:50:18.359 --> 00:50:20.920
how, you know, there can be variation
00:50:21.079 --> 00:50:23.799
in that, in those nutrients.
00:50:23.520 --> 00:50:26.079
That makes sense when you say it, but we don't think that.
00:50:26.079 --> 00:50:27.280
No, no.
00:50:27.280 --> 00:50:30.240
I mean, to me it's just crazy, but but it doesn't
00:50:30.400 --> 00:50:32.320
you know but but maybe it's not crazy
00:50:32.480 --> 00:50:34.400
because that plant is a living thing
00:50:34.560 --> 00:50:35.520
too.
00:50:35.340 --> 00:50:35.740
Right.
00:50:35.740 --> 00:50:37.660
And it's got to balance its
00:50:37.900 --> 00:50:38.700
needs.
00:50:38.700 --> 00:50:43.420
It's all about, I think a lot of it can be boiled down to trade-offs.
00:50:43.760 --> 00:50:44.079
So
00:50:44.640 --> 00:50:48.320
a plant has to put its energy into growth, so
00:50:48.560 --> 00:50:48.800
be its
00:50:48.960 --> 00:50:50.880
leaves and how tall it gets, or
00:50:51.119 --> 00:50:52.480
reproduction, its
00:50:52.880 --> 00:50:54.880
pollen and nectar.
00:50:54.880 --> 00:50:55.200
And
00:50:56.099 --> 00:51:00.500
It's kind of I'm very simp I'm simplifying this a lot, but it's kind of a choice.
00:51:00.500 --> 00:51:03.460
How are you gonna budget those nutrients
00:51:03.720 --> 00:51:04.039
And
00:51:04.359 --> 00:51:13.160
if it's not to the pollen and the nectar, and they don't actually have to be budgeted the same, you know, you might have really good nectar, but not great pollen.
00:51:13.320 --> 00:51:14.760
All of it's a balance.
00:51:14.760 --> 00:51:20.600
And so that's all affected by the different aspects of the environment that the plant is experiencing.
00:51:20.600 --> 00:51:21.960
It's interesting because even with
00:51:22.120 --> 00:51:22.760
just with that,
00:51:23.000 --> 00:51:25.880
I was just reading an older text while Frank Pellet.
00:51:25.660 --> 00:51:26.780
American honey plants
00:51:26.940 --> 00:51:28.380
and basswood, I think
00:51:28.540 --> 00:51:32.300
it was it two to three great crops every five years or something like that.
00:51:32.300 --> 00:51:32.620
So
00:51:32.940 --> 00:51:33.660
so we've like
00:51:33.820 --> 00:51:34.940
known it, but not
00:51:35.240 --> 00:51:35.560
known
00:51:35.720 --> 00:51:36.040
it.
00:51:37.000 --> 00:51:37.160
So
00:51:37.320 --> 00:51:39.880
it takes scientists like you all to like
00:51:40.040 --> 00:51:40.440
help us
00:51:40.600 --> 00:51:41.800
help us get there.
00:51:41.800 --> 00:51:42.280
Yeah.
00:51:42.280 --> 00:51:43.000
Yeah.
00:51:43.240 --> 00:51:44.600
Yeah, absolutely.
00:51:44.600 --> 00:51:46.120
I think it's really important
00:51:46.280 --> 00:51:48.280
that beekeepers understand
00:51:48.600 --> 00:51:50.280
the work that you're doing.
00:51:50.280 --> 00:51:52.440
It's it's it's honestly so revolutionary
00:51:52.600 --> 00:51:53.160
because right
00:51:53.320 --> 00:51:54.200
we used to
00:51:54.440 --> 00:51:56.520
measure the health of the colony by maybe
00:51:56.680 --> 00:51:56.920
the
00:51:57.079 --> 00:51:58.839
the amount of honey it puts up.
00:51:58.839 --> 00:51:59.720
But you're going
00:51:59.880 --> 00:52:01.079
into the organism
00:52:01.560 --> 00:52:01.880
and
00:52:02.320 --> 00:52:03.600
understanding what
00:52:04.240 --> 00:52:05.200
the actual
00:52:05.440 --> 00:52:09.200
health of the bee is and then connecting that to the health of the colony
00:52:09.440 --> 00:52:11.760
and helping us to interpret
00:52:11.859 --> 00:52:13.700
colony health, colony death, all of that.
00:52:13.700 --> 00:52:15.859
But but the work that you're doing inside the bee
00:52:16.020 --> 00:52:16.099
is
00:52:16.260 --> 00:52:17.380
is so critical.
00:52:17.380 --> 00:52:19.300
Can you each just comment on that?
00:52:19.300 --> 00:52:19.460
And
00:52:19.700 --> 00:52:22.579
'cause it's it's not like we've been able to do this
00:52:22.740 --> 00:52:23.859
for years and years.
00:52:23.859 --> 00:52:25.700
This is this is kind of a newer
00:52:26.220 --> 00:52:26.619
newer
00:52:26.859 --> 00:52:27.819
field for
00:52:28.140 --> 00:52:29.420
science and for
00:52:29.579 --> 00:52:30.859
livestock, isn't it?
00:52:30.859 --> 00:52:31.900
Or for honey bees?
00:52:32.220 --> 00:52:36.380
Deep in that gut there's a lining of microbes.
00:52:36.240 --> 00:52:40.320
There's also a lining of microbes throughout the entire colony.
00:52:40.320 --> 00:52:44.400
There's microbes on the honey, not that many.
00:52:44.440 --> 00:52:46.280
Because that's an age of the honey.
00:52:46.280 --> 00:52:46.680
But there are
00:52:46.839 --> 00:52:49.000
microbes on the bee bread.
00:52:49.320 --> 00:52:49.560
Also
00:52:49.960 --> 00:52:51.800
not that many, right?
00:52:51.800 --> 00:52:55.480
There's microbes on the cuticle of the worker bee.
00:52:55.359 --> 00:52:55.520
and
00:52:55.680 --> 00:52:57.520
microbes all over its face
00:52:57.760 --> 00:52:58.079
and
00:52:58.240 --> 00:53:01.440
microbes on the queen's face and mouth parts.
00:53:01.440 --> 00:53:01.520
And
00:53:01.760 --> 00:53:04.079
microbes everywhere in that colony.
00:53:04.079 --> 00:53:05.119
And one of the
00:53:05.280 --> 00:53:05.440
uh
00:53:05.599 --> 00:53:07.760
so this is a very new field
00:53:07.920 --> 00:53:08.720
and what you
00:53:08.960 --> 00:53:10.160
as you mentioned.
00:53:10.140 --> 00:53:12.460
And what happens with a new field is
00:53:12.619 --> 00:53:13.099
is uh
00:53:13.260 --> 00:53:14.539
well the process of science
00:53:14.700 --> 00:53:14.779
is
00:53:15.020 --> 00:53:15.579
edit
00:53:15.819 --> 00:53:16.779
and refine
00:53:16.940 --> 00:53:19.500
and edit and refine, right?
00:53:19.559 --> 00:53:24.119
It seems to be the process of society in general, but it's also the process
00:53:24.359 --> 00:53:25.640
of science.
00:53:25.640 --> 00:53:25.960
And
00:53:26.680 --> 00:53:28.920
so what is happening is we're in a big
00:53:29.079 --> 00:53:29.400
uh
00:53:29.740 --> 00:53:29.980
I f
00:53:30.140 --> 00:53:32.220
I feel like I'm in a big refine
00:53:32.460 --> 00:53:33.340
moment, right?
00:53:33.340 --> 00:53:34.460
Or big edit.
00:53:34.460 --> 00:53:35.020
Well yeah,
00:53:35.820 --> 00:53:36.300
produce
00:53:36.860 --> 00:53:37.340
produce
00:53:37.580 --> 00:53:38.300
produce has
00:53:38.460 --> 00:53:39.740
gotta be in there somewhere.
00:53:39.740 --> 00:53:42.140
Produce, edit, refine, right?
00:53:41.859 --> 00:53:45.300
It's the thesis, the antithesis, and the synthesis.
00:53:45.300 --> 00:53:47.540
So I guess there's gotta be three of them, right?
00:53:47.540 --> 00:53:47.700
So
00:53:47.859 --> 00:53:52.020
I'm just making up words here that other people have said better than me, but
00:53:52.080 --> 00:53:56.000
Yeah, I'm in a process of like, well, a lot of garbage has spun
00:53:56.160 --> 00:53:57.600
forth out of the microbial
00:53:57.760 --> 00:53:58.400
world.
00:53:58.400 --> 00:53:59.600
A whole lot of it.
00:53:59.600 --> 00:54:03.200
And it's it's it's fueling a lot of speculation about
00:54:03.540 --> 00:54:06.580
making probiotics and it's it's creating well that
00:54:06.740 --> 00:54:11.940
this, you know, false hope is the same as hope, but that's where, you know, this is where things come from.
00:54:11.940 --> 00:54:12.260
So
00:54:12.460 --> 00:54:12.700
It's
00:54:12.860 --> 00:54:19.100
uh but you know, it should narrow out and then through the editing process maybe we will find something that works.
00:54:19.100 --> 00:54:24.380
But right now it's it's about shedding all of the garbage off of the field.
00:54:23.900 --> 00:54:27.339
So there's an incredible amount of garbage in the microbial
00:54:27.500 --> 00:54:28.220
field now.
00:54:28.220 --> 00:54:28.859
It's uh
00:54:29.099 --> 00:54:29.500
I'm
00:54:29.740 --> 00:54:33.099
writing a paper about that presently, but uh
00:54:33.700 --> 00:54:35.540
And it's due to a lot of
00:54:35.860 --> 00:54:37.700
lot of different things, primarily
00:54:37.940 --> 00:54:38.900
misunderstanding
00:54:39.220 --> 00:54:41.060
how to do the analysis.
00:54:41.060 --> 00:54:41.780
And it's a
00:54:41.940 --> 00:54:42.740
it's a cut and
00:54:42.900 --> 00:54:43.540
paste
00:54:43.940 --> 00:54:47.380
thing now where you can send your samples away to a lab and you get them
00:54:47.540 --> 00:54:53.700
back and then you can choose little default parameters about how to analyze them and you go click click click click
00:54:53.859 --> 00:54:55.940
and then it spits out a thing.
00:54:55.400 --> 00:54:58.760
And then you look up those microbes and learn something about them
00:54:58.920 --> 00:55:00.039
and write it down.
00:55:00.039 --> 00:55:00.920
And it's like a
00:55:01.079 --> 00:55:04.920
it's like a plug and play or, you know, cut and paste.
00:55:04.760 --> 00:55:05.160
And is
00:55:05.320 --> 00:55:06.440
these people haven't really
00:55:06.600 --> 00:55:10.760
and maybe it's the nature of a lot of things now, but perhaps they haven't earned the
00:55:11.160 --> 00:55:11.800
the right
00:55:12.040 --> 00:55:14.040
to look at that data.
00:55:14.460 --> 00:55:14.940
And it
00:55:15.500 --> 00:55:15.740
it may
00:55:17.980 --> 00:55:21.180
it may be true of a lot of things, but you go through
00:55:21.340 --> 00:55:25.180
there's a lot of loops you go through to try to learn that stuff.
00:55:24.960 --> 00:55:26.240
Don't don't put that on.
00:55:26.240 --> 00:55:26.880
That's horrible
00:55:27.040 --> 00:55:28.000
thing you say.
00:55:28.880 --> 00:55:31.600
I don't want to discourage anybody, but
00:55:31.760 --> 00:55:32.000
uh
00:55:32.160 --> 00:55:32.880
yeah.
00:55:32.880 --> 00:55:35.680
I mean if I can just jump in here, I think
00:55:35.940 --> 00:55:40.260
I think you're just talking about one very small layer of the onion.
00:55:40.260 --> 00:55:40.820
So we're just
00:55:40.980 --> 00:55:44.980
we're just peeling back all these different layers of the onion.
00:55:45.020 --> 00:55:45.579
Right?
00:55:45.579 --> 00:55:45.900
And
00:55:46.060 --> 00:55:51.740
you get to go into a new layer of onion when you develop new methods and techniques.
00:55:51.740 --> 00:55:52.380
Right?
00:55:52.380 --> 00:55:56.940
And that helps you understand a very specific layer of the onion.
00:55:56.840 --> 00:55:57.240
Right?
00:55:57.240 --> 00:56:00.280
And then there's going to be a new layer and a new layer.
00:56:00.280 --> 00:56:05.000
All of that needs to come together to help us understand
00:56:05.160 --> 00:56:08.040
how not only the colony functions.
00:56:08.120 --> 00:56:11.160
the individual functions, but then even
00:56:11.480 --> 00:56:15.320
within the individual, you know, how that all functions.
00:56:15.320 --> 00:56:15.640
So
00:56:15.880 --> 00:56:19.240
so all of these layers, it is revolutionary
00:56:19.560 --> 00:56:19.880
because
00:56:20.420 --> 00:56:20.660
We
00:56:20.980 --> 00:56:21.300
have
00:56:21.460 --> 00:56:24.340
all these new things to measure and understand.
00:56:24.340 --> 00:56:26.420
However, you can't get
00:56:26.660 --> 00:56:28.820
you can't get like distracted
00:56:29.140 --> 00:56:31.700
by all the onion layers, right?
00:56:32.040 --> 00:56:33.080
And you have to
00:56:33.240 --> 00:56:36.280
I mean it's really easy to get distracted, right?
00:56:36.280 --> 00:56:38.600
And just really get in, you know.
00:56:38.680 --> 00:56:40.760
Was I getting distracted?
00:56:41.480 --> 00:56:43.720
No, no.
00:56:43.720 --> 00:56:45.000
Absolutely not
00:56:45.420 --> 00:56:45.660
But
00:56:45.900 --> 00:56:50.620
you really have to, you know, working for the ARS, I can only speak to that
00:56:50.780 --> 00:56:51.100
really
00:56:51.500 --> 00:56:52.940
with bees, our
00:56:53.180 --> 00:56:54.620
job is slightly different
00:56:54.860 --> 00:56:55.180
because
00:56:55.300 --> 00:56:57.540
Our job is to help agriculture.
00:56:57.540 --> 00:57:08.980
And so you have to always be focusing on the layers and the aspects of the layers that eventually are going to make colonies happier, colonies healthier, and help crop pollination.
00:57:08.380 --> 00:57:12.140
So there's a lot of stuff out there, but you really have to focus.
00:57:12.300 --> 00:57:15.180
Vanessa, I don't like onions, but I like your medicine
00:57:15.580 --> 00:57:15.660
right
00:57:15.900 --> 00:57:16.140
there.
00:57:18.480 --> 00:57:20.480
It's it's you know, when I was
00:57:20.640 --> 00:57:25.280
I will say when I was young, when I was young and started into this, you know, and
00:57:25.520 --> 00:57:27.040
always seemed to be that uh
00:57:27.200 --> 00:57:27.920
my friends at
00:57:28.400 --> 00:57:28.960
bees uh
00:57:29.120 --> 00:57:29.760
needed
00:57:29.740 --> 00:57:29.980
help
00:57:30.140 --> 00:57:32.140
about the time that they were harvesting honey
00:57:32.300 --> 00:57:32.780
and you
00:57:33.020 --> 00:57:34.620
you probably could guess why.
00:57:34.620 --> 00:57:37.020
The one thing about it was you know back then
00:57:37.180 --> 00:57:40.460
looked at it and you see the wealth of it was in the honey in the
00:57:40.559 --> 00:57:42.880
the frames and stuff like that and you're missing the it
00:57:43.039 --> 00:57:43.200
it's
00:57:43.359 --> 00:57:44.640
misses the whole point.
00:57:44.640 --> 00:57:46.640
It's having healthy colonies that
00:57:46.799 --> 00:57:50.000
where the true wealth of uh beekeeping is.
00:57:49.840 --> 00:57:52.480
If you can do that, the rest of it follows uh
00:57:52.640 --> 00:57:58.720
through, especially in this time when everything's coming at these colonies and destroying them.
00:57:58.559 --> 00:58:04.640
Yeah, I think if people even tried to work in a particular part of the field, they can't ignore these other parts.
00:58:04.640 --> 00:58:06.160
You know, folks who work on
00:58:06.359 --> 00:58:08.760
Pathogens have to think about what pesticides
00:58:08.920 --> 00:58:11.800
exposures are going to alter things there.
00:58:11.800 --> 00:58:13.320
The nutrition parts, uh
00:58:13.480 --> 00:58:15.720
looking at forage and nutrition.
00:58:15.559 --> 00:58:18.039
Well, what if you get exposed to something um
00:58:18.200 --> 00:58:23.559
like a strong pesticide when your colony is weak and is going to get taken out by it
00:58:23.540 --> 00:58:23.700
It
00:58:23.860 --> 00:58:24.340
just kind of
00:58:24.500 --> 00:58:26.260
can't put down all the parts and it
00:58:26.420 --> 00:58:28.420
that's why we always seem to inevitably
00:58:28.580 --> 00:58:31.540
end up working on several different things at once
00:58:31.180 --> 00:58:32.140
But it makes for Lance
00:58:32.380 --> 00:58:33.420
collaboration.
00:58:33.420 --> 00:58:41.660
And that's one of the reasons why the honey bees is one of the what the most studied insect in the world, so it's so fascinating on so many different levels, so many different
00:58:41.900 --> 00:58:43.180
peels of the onion.
00:58:43.180 --> 00:58:44.860
To beat that metaphor again
00:58:45.140 --> 00:58:46.339
Well, I will have to say
00:58:46.500 --> 00:58:49.380
this has been a total pleasure, worth the wait.
00:58:49.380 --> 00:58:52.579
I enjoyed talking with you all and I look forward to having you back.
00:58:52.579 --> 00:58:53.380
I would I
00:58:53.539 --> 00:58:56.900
I'd almost like to say let's do this on a semi-annual basis.
00:58:56.900 --> 00:58:59.859
Every six months we'll just uh kind of circle back.
00:58:59.840 --> 00:59:01.520
And I will break that cycle.
00:59:01.520 --> 00:59:01.680
Uh
00:59:01.840 --> 00:59:02.480
I will
00:59:02.880 --> 00:59:03.280
we're w
00:59:03.440 --> 00:59:05.120
uh just a little sneak peek.
00:59:05.120 --> 00:59:07.680
I will be in Tucson in November for other events.
00:59:07.680 --> 00:59:09.440
We're we're gonna try to get into
00:59:09.600 --> 00:59:10.000
the B
00:59:10.240 --> 00:59:10.400
lab
00:59:10.640 --> 00:59:10.720
for
00:59:10.880 --> 00:59:13.360
a visit and uh a field report
00:59:13.200 --> 00:59:14.560
So I'm looking forward to that.
00:59:14.560 --> 00:59:14.720
But
00:59:14.880 --> 00:59:21.520
beside that, maybe next spring we can have you back and we can talk about future research and some of the things that you're looking forward to.
00:59:21.760 --> 00:59:23.200
I think that would be exciting.
00:59:23.440 --> 00:59:24.560
Absolutely.
00:59:24.380 --> 00:59:24.700
Thank you.
00:59:24.860 --> 00:59:26.940
Yeah, we can talk about bee research all day.
00:59:26.940 --> 00:59:27.180
So
00:59:27.580 --> 00:59:27.740
the
00:59:27.900 --> 00:59:30.460
three-hour talk, it's coming up right now.
00:59:31.580 --> 00:59:34.380
Well thank you so much and we look forward to having you back.
00:59:34.380 --> 00:59:34.700
Thank you.
00:59:35.580 --> 00:59:35.900
Thank you.
00:59:36.380 --> 00:59:36.780
Take care.
00:59:37.100 --> 00:59:39.260
Thank you.
00:59:39.700 --> 00:59:42.180
I don't know how many times I can say this during an episode
00:59:42.340 --> 00:59:47.300
and I'm sure there's folks out there who are counting it, but I really enjoyed having them on the show.
00:59:47.300 --> 00:59:49.300
I knew when I met them in June
00:59:49.540 --> 00:59:51.300
that they would be fun to have on the
00:59:51.540 --> 00:59:52.340
podcasting
00:59:53.040 --> 00:59:55.040
They met all expectations.
00:59:55.040 --> 00:59:56.000
Oh, you were so
00:59:56.160 --> 00:59:57.280
you were so right.
00:59:57.280 --> 01:00:00.320
That was just a jam-packed conversation.
01:00:00.320 --> 01:00:06.320
There was so much information, so many layers that we still could peel back.
01:00:06.040 --> 01:00:07.560
and explore.
01:00:07.560 --> 01:00:10.920
It's just a new road that research is taking.
01:00:10.920 --> 01:00:11.240
I
01:00:11.400 --> 01:00:12.600
I think for last
01:00:12.840 --> 01:00:14.040
early last year, I r
01:00:14.280 --> 01:00:17.400
oh for February, because it was blood related, I
01:00:17.740 --> 01:00:17.820
r
01:00:18.140 --> 01:00:19.100
really, which is not
01:00:19.260 --> 01:00:21.660
called blood, it's insect hemolymph.
01:00:21.660 --> 01:00:24.300
But I wrote an article for Bee Culture
01:00:24.860 --> 01:00:25.180
about
01:00:25.740 --> 01:00:27.420
biomarkers in Hemolymph
01:00:27.580 --> 01:00:34.860
and how many different avenues there are now to actually measure the health of bees through biomarkers, which
01:00:35.180 --> 01:00:35.500
really
01:00:35.740 --> 01:00:39.180
gives a researcher so many more tools
01:00:39.220 --> 01:00:39.460
to
01:00:39.779 --> 01:00:40.660
analyze
01:00:40.980 --> 01:00:43.140
colonies and colony health.
01:00:43.140 --> 01:00:43.380
So
01:00:43.619 --> 01:00:48.819
to talk to three people who are kind of doing some work around there, it's it's
01:00:48.980 --> 01:00:49.299
that was
01:00:49.460 --> 01:00:50.420
exciting.
01:00:50.240 --> 01:00:52.560
They're right in the mix of all the excitement
01:00:52.720 --> 01:00:54.720
and they're connected around the world.
01:00:54.720 --> 01:00:56.080
I'm looking forward to having them back.
01:00:56.080 --> 01:00:57.680
I'm looking forward to talking to them hopefully
01:00:57.840 --> 01:01:00.400
in November and then again next spring
01:01:00.400 --> 01:01:01.040
we can
01:01:01.200 --> 01:01:03.360
take a deeper dive into something.
01:01:03.360 --> 01:01:05.040
I'm sure we'll find something.
01:01:05.040 --> 01:01:06.480
I'd love these USDA
01:01:06.640 --> 01:01:07.360
researchers.
01:01:07.360 --> 01:01:09.040
We've got Jay Evans.
01:01:09.040 --> 01:01:09.280
We
01:01:09.440 --> 01:01:11.520
just talked to Vince and these three
01:01:11.680 --> 01:01:12.000
areas.
01:01:12.240 --> 01:01:12.560
Vanessa
01:01:12.720 --> 01:01:13.920
Kirk and Mark, yeah.
01:01:13.920 --> 01:01:14.880
There you go.
01:01:15.000 --> 01:01:15.240
And
01:01:15.480 --> 01:01:19.080
that about wraps it up for this episode of Beekeeping Today.
01:01:19.080 --> 01:01:22.440
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01:01:22.600 --> 01:01:26.680
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01:01:31.680 --> 01:01:37.920
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01:01:37.920 --> 01:01:43.040
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01:01:43.040 --> 01:01:44.960
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01:01:45.240 --> 01:01:49.160
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01:01:49.320 --> 01:01:52.360
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01:01:52.360 --> 01:01:56.280
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01:01:56.280 --> 01:01:59.880
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01:02:00.320 --> 01:02:00.640
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01:02:00.640 --> 01:02:02.000
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01:02:02.000 --> 01:02:03.920
Thanks again, everybody.
PhD, Research Entomologist
I am a Research Entomologist at the USDA-ARS Carl Hayden Bee Research Center in Tucson, Arizona. I received my BS in Natural Sciences from New College of USF and my MS and PhD in Entomology from University of Illinois with Dr. May Berenbaum. I worked with Dr. Eric Schmelz of USDA-ARS CMAVE on the basics of how plants detect attacks before joining the world of honey bees in 2006.
My research focuses on how colony stressors (poor nutrition/forage, parasites, pathogens, overwintering/seasonal stress, and chemical treatments) disrupt colony cohesion, communication, and performance, and the development of practical solutions to reduce these effects. Honey bees thrive worldwide through considerable flexibility and adaptation under a wide range of conditions. Many stressor effects impact key colony functions, such as queen maintenance, brood production, and nutrition, that are necessary for colony performance and survival. I am particularly interested in improving queen retention, overwintering bee nutrition and productivity, and Varroa mite control to promote colony health and performance. A better understanding of how bees respond to stress and how we can work within these confines is a path to improved beekeeping.
Ph.D., Research Insect Physiologist
Dr. Vanessa Corby-Harris obtained her B.S. in Animal Science from North Carolina State University in 2001 and her Ph.D. in Genetics in 2007 at the University of Georgia. In 2011 she joined the USDA Carl Hayden Bee Research Center as a postdoctoral scientist. She was hired as a Research Insect Physiologist in 2015. Drawing on her expertise in other insect and non-insect systems, her research seeks to improve colony health through better nutrition. Her main questions are: 1) What nutrients do honey bees need? 2) Do these nutritional needs change depending on season, location, stress, or other variables? 3) How can we make sure that honey bees get these nutrients through supplemental diets or natural forage?
https://corbyharrislab.weebly.com/
USDA Honey bee Scientist
Dr. Kirk E. Anderson has conducted research in the ecology and evolution of social insects for 25 years and his present focus is honey bee microbial ecology. He is Lead Scientist of the active 5-year research plan “The Honey Bee Microbiome in Health and Disease”, at the Carl Hayden Bee Research Center in Tucson AZ. His research is aligned with the Action Plan for ARS National Program 305 – Crop Production.
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